FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_F01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_F01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146010
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT37740.32931649811083674No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG31630.27600108201499113No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG26860.23437840856537026No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA25550.22294744373958345No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG23540.20540832977024634No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT23490.2049720334028499No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA23390.204099440668057No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC23000.2006963290023647No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA19710.17198802802767865No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT18850.1644837305084598No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA15410.13446654043158437No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC15090.13167424368024713No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG14680.12809661346759627No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC13570.11841083411139519No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG13560.11832357483791589No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC13430.11718920428268513No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA13190.11509498171918221No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA13180.11500772244570291No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT12840.1120409071474071No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA12580.10977216603694558No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC11880.10366401689339536No Hit
ATATATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAAT11710.10218060924424743No Hit
GTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTATTATTATT11670.10183157215033027No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA11510.10043542377466165No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)