FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_E07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_E07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735554
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA16760.227855466763827No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG14010.1904686807494759No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA12070.1640940026157155No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG11980.16287043507342763No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA11760.15987949219227957No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA11160.15172237524369386No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA10240.13921479592252914No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG9920.13486433354995012No Hit
TCCCAGGACCCTGGGATCATTACCTGAGCCAAAGGCAGATGCCCAACAAC9200.12507579321164727No Hit
CCACAGGGCCAGTCCCTGAGTCTGTCACATACACAGCAATACAAGCTTCT8890.12086128278821133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)