FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_E05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_E05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544462
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAGAAGTTGCATAAGTACGATTATAAAAAGTATACCCACTTTTGTC16740.30745947375574423No Hit
CTTCTAAACATTGAAAAAAATTTTTTTAGATTAACTTGTGTGCAGTGTCA15030.2760523232108026No Hit
CTATGTATGTTTAACACAATTACCTCTCAATATATCAATCTCTGCCTACT14560.2674199484996198No Hit
GTATGTGAAGTATATTCTCCAAAAATAAGCACTTTTGACCAAAGTTTATT13180.24207382700721078No Hit
CTGCAGGACTGGTTGCTAGAACTCAAATCCCGTTAACTTCCTTCTGTGAT12010.22058472400277707No Hit
CTTCACATACTATGTATGTTTAACACAATTACCTCTCAATATATCAATCT11800.21672770551480178No Hit
CCCTTGAACATCTCTTCATATATTTGAGCCCTTTTTCATTTCTTTTTCTA11480.21085034400931563No Hit
CATACATAGTATGTGAAGTATATTCTCCAAAAATAAGCACTTTTGACCAA11310.2077279957095261No Hit
ATCTAAGGGTATCTTCTAAACATTGAAAAAAATTTTTTTAGATTAACTTG10880.19983029118652906No Hit
GCCTCAAGGAGCTGTAGGGTTGTTATGACAAGAATAACAAGAGAACACTC10450.19193258666353208No Hit
CTTCATATATTTGAGCCCTTTTTCATTTCTTTTTCTAAGAAAAAGGCCTC10380.19064691383420698No Hit
ATATCAATCTCTGCCTACTTTTCCAACCACTCACAGATGTCTTTCCCTCA10330.18972857609897475No Hit
ACCTACAGGTCATAACCAGACAGCTCTTACTGTGAGGACCATAATCCACA10210.18752456553441746No Hit
GTGTCAGCATACACCATTTTGTGGACAACCTACAGGTCATAACCAGACAG10120.18587155761099947No Hit
TAATACGAGTTCCCTTGAACATCTCTTCATATATTTGAGCCCTTTTTCAT9340.171545488941377No Hit
CAATATATCAATCTCTGCCTACTTTTCCAACCACTCACAGATGTCTTTCC9330.17136182139433054No Hit
GTCCTGTAATGTGGAGCTGGAAATAGAAGAGAGGAAGAATGTGACAGTCC8790.16144377385382266No Hit
GTAAAAATCTGTCCTGTAATGTGGAGCTGGAAATAGAAGAGAGGAAGAAT8490.1559337474424294No Hit
TAACTAGACTGTCACAAAGCCGCTTGCCACTGGGTGAGAGAGGCACCAGC8150.1496890508428504No Hit
TTACAGGACAGATTTTTACTCTGCTTTTATTTTGCTTTTTCTTCTCATAT8120.14913804820171106No Hit
GAATAACAAGAGAACACTCATTGTTCAATTTTTTATGACTTCTGAAAGAA7850.1441790244314571No Hit
CTCTAGAATCCTGCCCTCACTGCCAGAGGCAGTGGGAGACCCAGATCCCG7800.1432606866962249No Hit
AAAGCAGGCTGAGCATGGAGCTTGATGTGAGGCTTGATTTCACAACCCTG7280.1337099742498099No Hit
GTATATAGTCAAGGGCTCGATGGTAATTCCAAACTCGACTCTGGAACTGA7220.13260796896753124No Hit
GAATGTGACAGTCCCATGCTACTTGCCCCGGGTGAGTGCGGCTGACGGAT7120.13077129349706684No Hit
GTCCCATGCTACTTGCCCCGGGTGAGTGCGGCTGACGGATGAGTGCAGCT7050.12948562066774175No Hit
TTCTTAAACAGGTCCCCCAAAACTGGCAGAATAAGCACATTACTTAGCAA6910.12691427500909153No Hit
GTGTAGGTTGAGGCTTTAAGCCCTCCTGTGCAAGTTTCATTGTCCATTAG6820.12526126708567356No Hit
GTAATGTGGAGCTGGAAATAGAAGAGAGGAAGAATGTGACAGTCCCATGC6630.12177158369179115No Hit
GTCTGTGAGGTTCATGCATTTATCTTCTCTTCCTCCTGAAATGGCACTAA6620.12158791614474472No Hit
ATAATAGCCTTGTGTGGTGACAAATGGTAGCCACACTTGCGGTGGGCATA6600.12122058105065182No Hit
ATCCCACACTGCAGGACTGGTTGCTAGAACTCAAATCCCGTTAACTTCCT6490.11920023803314098No Hit
CTGTTGGGAGTGTTCAGTTTTCTCAACTTTCTGTCATGATCCGCTGAAAG6470.11883290293904808No Hit
TTATTTACCCAAGTGTGTAGGTCTGCTTTTAGATGGGAAGGGAGATACTG6410.11773089765676943No Hit
GTTTAACACAATTACCTCTCAATATATCAATCTCTGCCTACTTTTCCAAC6390.11736356256267656No Hit
CCATAATCCACATCCTCCACAGTGAACATCTAGTGTGGAACTTTTAGATT6340.11644522482744434No Hit
CTTTGTGCCTGAGCATCAAGGCCTGACATTAATAACAATTTCTGGAAGAC6340.11644522482744434No Hit
GGTCAGTAGGTACTGACTGAACTACCCAGGTGTTAACTAGACTGTCACAA6330.1162615572803979No Hit
ATGTATGTTTAACACAATTACCTCTCAATATATCAATCTCTGCCTACTTT6290.11552688709221212No Hit
GTTCTCGGGTAGATTCAGGCCCAGCGTCCCTCTGCAAGCTGCAGGTTCGC6270.11515955199811925No Hit
CTCTTACTGTGAGGACCATAATCCACATCCTCCACAGTGAACATCTAGTG6270.11515955199811925No Hit
CTTTAAATATGAGCACAGTTTCGAAGAGTCAGATCTATCTACAAAACTTA6260.11497588445107279No Hit
GCTCTTACTGTGAGGACCATAATCCACATCCTCCACAGTGAACATCTAGT6190.1136902116217477No Hit
GTGTTAAACATACATAGTATGTGAAGTATATTCTCCAAAAATAAGCACTT6170.11332287652765483No Hit
GTCACATTCTTCCTCTCTTCTATTTCCAGCTCCACATTACAGGACAGATT6130.11258820633946905No Hit
ATATAGTCAAGGGCTCGATGGTAATTCCAAACTCGACTCTGGAACTGAAC6070.1114862010571904No Hit
GATATATTGAGAGGTAATTGTGTTAAACATACATAGTATGTGAAGTATAT6060.11130253351014395No Hit
CTTAATGACTGAGCCACCTAGGTGCCTCCATTTGGTAATTTTTAAAAATA6040.11093519841605107No Hit
GTTTCGAAGAGTCAGATCTATCTACAAAACTTATTTAGGAAATCAGCAGG6000.1102005282278653No Hit
GTTTTAATTTGAGTTTATCATAATACGAGTTCCCTTGAACATCTCTTCAT5920.10873118785149377No Hit
GAAGTATATTCTCCAAAAATAAGCACTTTTGACCAAAGTTTATTTGGTCA5770.10597617464579714No Hit
CCTCCACAGTGAACATCTAGTGTGGAACTTTTAGATTTCCTTGACAAGGA5730.10524150445761138No Hit
CTTCTATTTCCAGCTCCACATTACAGGACAGATTTTTACTCTGCTTTTAT5670.10413949917533272No Hit
GATTTTTACTCTGCTTTTATTTTGCTTTTTCTTCTCATATGTTTCTTTCA5590.10267015879896117No Hit
GATTGATATATTGAGAGGTAATTGTGTTAAACATACATAGTATGTGAAGT5550.10193548861077541No Hit
CTTGTGTGCAGTGTCAGCATACACCATTTTGTGGACAACCTACAGGTCAT5520.10138448596963609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)