FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_D12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_D12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCAAGAGTTGTATCCACTTCTAACTGAACCAGCCAGGCAAGCCTAG18.333333333333332No Hit
CCCGGGTGCTGGGATCATGACCTGAGGGGAAGGAAGACATTTAACGGACT18.333333333333332No Hit
CCCAAGTGATCGCTACTATTAAGTTGTTTCTAGGTGCTAGACATTGTGCC18.333333333333332No Hit
TGCACGCTGGCATGCACTAGCATGATCACAACTCACTGAAGCCTCAACCT18.333333333333332No Hit
TTCGTATTCCGCGTGGAGCGACTGATTCAAAGCCTGCCTGTCTCTTATAC18.333333333333332No Hit
AAACCATGTCATCCAAAATTATAGAAAAAACAACAGTTCTACAATATAAA18.333333333333332No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG18.333333333333332No Hit
GGTATCAACCGCCGAAGAGCCGCCTGCAGTGGGAATATAGCCTGCATAAA18.333333333333332No Hit
GATAAAAACAACTAAACAAAAAACTTTCATGAAAAAATGTCTTACTTGCA18.333333333333332No Hit
GATCCAGATGCAGGCTATAACCGCGCGCCAGACGAACCGCTTCTGGCAGG18.333333333333332No Hit
GGACCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCT18.333333333333332No Hit
TCTTAAATCTTCAAATGCAGCAGAGAGGCATGCCATCCACAACAATTGAC18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)