FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_D09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_D09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943571
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA33350.353444520868064No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG25830.27374728557787387No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG24570.2603937594521239No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA23080.2446026849065942No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA22150.2347465108613978No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG21230.2249963171822788No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA20840.22086308290526097No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA20440.21662386826216576No Hit
CCACAGGGCCAGTCCCTGAGTCTGTCACATACACAGCAATACAAGCTTCT15550.16479946925032668No Hit
GAGCAAAAGCAGGGGTAGGGGGCCTGTCAGCTCCATTTCCAGGGCCCCAT14910.15801672582137433No Hit
GAGCAAAAGCAGGCGGAGTAGGAGAGGTAGAAGCAGGCTTCCTGCCAAGC13980.14816055177617793No Hit
ATCCAGGCAGAGTGCACGGGTGTGTCACCGTGAATACTCAGGGAGCCGGA11880.12590467489992804No Hit
GTGTTACATTGTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTC11500.12187742098898759No Hit
CTCCCTAAAGGGACAAGATGTTATAAAAAGAAAGAAATACATTGACAGCG10740.11382291316710666No Hit
GTATTAAGGAGGATATCAGTGAGGTTTAAGTTTCAGATGTGTTTTGGGGA10600.11233918804202334No Hit
TCCCGGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA9980.10576840534522575No Hit
GTTCTATAGTGTTTGGATAAAAATAAGAAACAGAGAGGAGAGTCCAGCAC9930.10523850351483884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)