FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875342
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATGAGGGGACCTTCATTGAGAATGGCCAATGGGAAATTATCCACAAG20830.23796413287606447No Hit
GTATATAAGGCCATAAGATGTGAGGAGACAAGCTTTCGACAAACCTGTAA18460.21088900109899902No Hit
ATATAAGGCCATAAGATGTGAGGAGACAAGCTTTCGACAAACCTGTAACA17490.19980761805100175No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT15580.17798757514205876No Hit
TCCTAAGGGAGGCGGGGAAGGACAGCGCGAAGAAGTCACCTTCTACCTCA12960.14805641680623116No Hit
GAATAAAGGAAAGCCATCCAGGGCACTGAAGAAGGCACGTCAGGGGAATG11620.13274811445126591No Hit
CCCTAAGCGCACTGAGGGCAGAGCCCACACCTGATTGATCTTCTTGTTCT10830.12372307052557743No Hit
CTCCTTAACTATATAAATTCCAAGGGACATAACTGGGCCACACGGAGTCT9200.10510177736244805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)