FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_B10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_B10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075374
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG29730.276461956491416No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT22110.20560288792550313No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA16210.15073825478391703No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA14610.1358597102031479No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA13320.12386388363490282No Hit
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA13260.123305938213124No Hit
CTATAGAACAAAAATAAGAAAATCTTATCCATTGCCAGTGGTAGGACTCA12470.11595965682636926No Hit
CTTTGGTATGTTACACTTCAGTAAACATTTTACTGAAAATTAAAAAGAAG12000.11158908435576831No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG11430.10628860284886933No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA11400.10600963013797991No Hit
GTCACATACACAGCAATACAAGCTTCTCTACATTTCTAAACATTTTCTGG11240.10452177567990299No Hit
GTATTAAGGAGGATATCAGTGAGGTTTAAGTTTCAGATGTGTTTTGGGGA11210.10424280296901357No Hit
GAGCAAAAGCAGGGGTAGGGGGCCTGTCAGCTCCATTTCCAGGGCCCCAT11200.10414981206538376No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG11140.10359186664360492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)