FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_B08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_B08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980391
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACCGTACACAAAATTAAAAATGGATTAAGGACCTAGGGGCACC40440.41248848673641436No Hit
GTATTACACGTCACGATAAACGTGAATTGGGTGTGTTAACCGGGAAGAGG39480.4026964751818407No Hit
GGATAAAGGAGATGTCCATTATTTGTTCTCTCCACCATCCAGTGAAGCAT25600.2611203081219636No Hit
CCTCTAGGGTGCGCCTGTCCCTGAGTGCCAAGACTTATGAGCTAGATGGC23740.24214828573497718No Hit
GCTATATACAAAAGAATGAAACCGGACCAGTGTCTTATACCGTACACAAA22450.22899027020851886No Hit
GAATAAGATGGAGGGCCAAAGATGGAGTCAGTGTTTCAGAACTCCTAGCC21800.2223602623851096No Hit
GTTCTTAGGTGTGACAGAAGAAAATTAATGAACGTGCCCTTCCACATTTG21340.21766825684854307No Hit
CCTAAGAACTGGTGATGGGTAGTTTGGTTTCTGCCAGGGTCAGAGAGAGT21290.21715825624674237No Hit
ACCCTATGAGATACACATAATATGATCCCCATTTTGCAGTTAAGGAAACA20260.20665224384964773No Hit
TCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCTCTGGCTCCACTC20180.2058362428867666No Hit
GAAGAATATAGAGCCCAGAAATAAACCTGCAATTATATGGTCAATTAATC19900.20298023951668262No Hit
CCCTTAGACACTCTCCCCATCTCTAGGTCCTTCCTTATCTCTCCCTGGAG18820.1919642265177873No Hit
TCATAGGGTTGTGTGAGACTGAGGCAGTCAACATGTGTTAGGTAAGCACT17750.1810502136392521No Hit
GTCTGGGGCGGTCTGTGAGCAGGACCAGCTGGGCATGGAATGGGGCCCAC17060.1740122053344023No Hit
GGTAAGCACTGCGTGCATGTGGCTTAGTCCAGGAGGTAGGTAAGTAGTAG16790.17125820208467846No Hit
TCTCCGCACTTACTTCAAGGAGAACCAGGAGGTCCGGGAGTGGGATTTCC16320.1664641964277518No Hit
GTATAAAGACCTTTAAAAAAAAAAATGTTACATTAGTCACCACACAGTAC16290.16615819606667137No Hit
GTTCAGGGAATGGCCCTTGGTCATCCAAATGTGGAAGGGCACGTTCATTA15560.15871218728038097No Hit
GTCTAGGTACAGTCAACAGGATTTGCCCATCACGTGTGGGGTGAGAAAGA15240.15544818342885644No Hit
GCCCCAGACAGGTCCCCAGGGCAGGGCCCGGAAAGCAGGGCATCCTGGGT15190.15493818282705574No Hit
GTATTTATACATATTTCAGGGGAGGAGAATGGAAGCATGGGTGAGCCTGG15000.15300018054021305No Hit
GTTCTGCTCCTTCCCTCTGTGGGCCCCATTCCATGCCCAGCTGGTCCTGC14970.1526941801791326No Hit
GCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGTTTTGT14970.1526941801791326No Hit
CTTTTAATGATGGGCGATCCAGGAAGGACTTCTTGAAGTTAACTTTTGAG14900.15198017933661162No Hit
GTGCAGGTGGGTGTTCTTGTTCACGGTCAGGCTCCTGGCTGGACTCAGCA14090.1437181695874401No Hit
CTCTAGGTCCTTCCTTATCTCTCCCTGGAGAAATCCTTCTCCAGTTTCAA14070.14351416934671984No Hit
GAATTAGGGGGCTCTCTTTCCTCTTCCCGGTTAACACACCCAATTCACGT14010.14290216862455898No Hit
TATTATGTGTATCTCATAGGGTTGTGTGAGACTGAGGCAGTCAACATGTG13760.1403521656155554No Hit
GTCACAAGGATATCTGACAGAAGGGCACTCCTGAAAGATGGAACAGCAAG13750.14025016549519528No Hit
GTGGAGGAGATCCAAGGAAGGACAGTCACAGAATCAGGGAACGTCAGTTT13660.139332164411954No Hit
CATTTAAGGTGTGCTTCATGTAAGATCTAGCTTCTGGCTTTCTCCCTTAG13460.13729216200475117No Hit
CCTTAATGCTGCTTCTTGGCTACACATGGTCCCCATCAAAGTGACCCCAT13320.13586416031970916No Hit
GGCCTATACACCCCTAGGCTGGAGTTGCCATCAGTGAGAAGGGGAAGTAC13240.13504815935682804No Hit
ATCCTGGGTCATCCGACCATTCTGGATCAGAAACCGTGGGGCATGGTCTG13240.13504815935682804No Hit
CCCCTAGGCTGGAGTTGCCATCAGTGAGAAGGGGAAGTACATGGGGGTTA13160.13423215839394692No Hit
TTTCAGGACAGAAGGGAGCATCTCCGCACTTACTTCAAGGAGAACCAGGA13110.1337221577921462No Hit
CCCCTGCTCTCTACACTTCAAAGATTTCTGCTATTAGAATAAGGTTCTGT13000.13260015646818463No Hit
GTATAGGCCAGGAACCCAGGAGTCTCCAGCCTTCAGCCTTCTTTCTCACC12910.13168215538494335No Hit
GTCCAGGAGGTAGGTAAGTAGTAGTTGGATATGGGACGTGTTGTCTAGGT12480.12729615020945725No Hit
ACCAAGGGCCATTCCCTGAACCATGAGGGCATGGGAAGGCTGCCATAGCT12450.12699014984837684No Hit
TGCTTGTACATTCCAGGGTTACAAGGAGCTTGGGGCTGGAGCCAGAGGGA12380.12627614900585582No Hit
GAATAAGGTTCTGTTTTGTTAAGGTGGCACAGGAGGTCCTTTAAACCTAA12370.12617414888549566No Hit
CATCAAAGGAGGCAAGAATATGCAATGGGGGGAAAAAAGTCTCTCCAACA12290.12535814792261454No Hit
CATAATATGATCCCCATTTTGCAGTTAAGGAAACAGGCCCAGAGTGGTCA12030.12270614479325086No Hit
CCCATGCATCCTGCAATTTGTTAGTTTTGCTGTTGCTTAGCAAGACATTC12020.12260414467289071No Hit
GAAGGGAGCATCTCCGCACTTACTTCAAGGAGAACCAGGAGGTCCGGGAG11990.1222981443118103No Hit
ATCTATAACAAATACATCAAAGGAGGCAAGAATATGCAATGGGGGGAAAA11770.12005414166388717No Hit
GTGTGAGACTGAGGCAGTCAACATGTGTTAGGTAAGCACTGCGTGCATGT11690.11923814070100604No Hit
GTAATAAGGAGAACACATTTTGCAACGAGCACTGGATGTTATATGCAAAC11620.11852413985848503No Hit
ATTAAGATGTGAGCAGAAGACTGATGTGACATGACCCATGGGAACTAAGT11600.11832013961776476No Hit
CTCTGAGGGTCCGCAGCACCATCTCGAGTCTGTGGTCATATCCACAAGAA11600.11832013961776476No Hit
GCCTACTTGTGATCTCTGTCTGTCAAATAAATAAATAAAATCTTAAAAAA11440.11668813769200248No Hit
GTGCTTACCTAACACATGTTGACTGCCTCAGTCTCACACAACCCTATGAG11430.11658613757164232No Hit
CTCCTGAAAGATGGAACAGCAAGTGCAAAGGCCCCGGAGCTGTACTCCTG11410.11638213733092205No Hit
GTCTGGCGCTCCATCCCCTTCTCTTACAACGAAAATGGGACCTGCCACTC11370.11597413684948149No Hit
GTACATGGGGGTTAGGGAGCAGGTCTGGTTAGTAGAGGAATTTAGACTCA11340.11566813648840105No Hit
ATCCAGGAAGGACTTCTTGAAGTTAACTTTTGAGCAGAGCCCTGAAGGAG11230.1145461351644395No Hit
CTCTGGGCCTGTTTCCTTAACTGCAAAATGGGGATCATATTATGTGTATC11120.11342413384047792No Hit
GTCTCACACAACCCTATGAGATACACATAATATGATCCCCATTTTGCAGT11100.11322013359975766No Hit
CTCTCTGACCCTGGCAGAAACCAAACTACCCATCACCAGTTCTTAGGTGT11020.1124041326368765No Hit
GGCCCAGAGTGGTCACATGGCTCGTAACTGGTCCCCTGCTTTCCTCTTTA10940.11158813167399537No Hit
ACATTGGGCTCTCTGCTCAGCAGGGAGCCTTCTTCCTCCTCTCTCTCTCT10820.11036413022967367No Hit
GTCTTCTCTTGAGTTAATGGCCAGTCTGGCGCTCCATCCCCTTCTCTTAC10530.10740612673922954No Hit
CCTTAAGGGGACACCCAAGAAGTTTACTGGAAGCATGAAGGGGAAATTTG10510.10720212649850927No Hit
GTCCTGGGATCGAGCCCCACATTGGGCTCTCTGCTCAGCAGGGAGCCTTC10410.10618212529490785No Hit
ACCCAGGATGCCCTGCTTTCCGGGCCCTGCCCTGGGGACCTGTCTGGGGC10400.10608012517454771No Hit
GGACATGATCTCAGGGTCCTGGGATCGAGCCCCACATTGGGCTCTCTGCT10360.10567212469310713No Hit
CACATAATATGATCCCCATTTTGCAGTTAAGGAAACAGGCCCAGAGTGGT10230.10434612312842528No Hit
AGAATATAGAGCCCAGAAATAAACCTGCAATTATATGGTCAATTAATCTA9950.10149011975834131No Hit
CCCTAATTCAGAGGGGCTCTGAGAAAGTGGGGAGCTCTAGAGGAAACACG9910.10108211927690074No Hit
GTTACAAGGAGCTTGGGGCTGGAGCCAGAGGGAGTGAGGCCTGGAATGAG9840.10036811843437976No Hit
CCCTAATGGTTATCGACATTGCTCCGGAGCAGTAGACCCTATCAAGGACA9820.10016411819365946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)