FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S17_R1_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S17_R1_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680437
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA22970.3375771746686321No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT20730.304657154152405No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG17000.24983944141779474No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG12880.18929011796830567No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT12650.1859099372903002No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA11470.16856814076835916No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC10990.16151385065773907No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG10710.15739884809321067No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC8970.13182704644221285No Hit
CCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTTTTTTCA8940.13138615331029913No Hit
ATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAACATCG8660.12727115074577072No Hit
GAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAA8380.12315614818124235No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA8210.1206577537670644No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG8100.11904114561671396No Hit
GTATAACTGGAATCCAGAAGGAAGGAAAGACAGAAGAAAATATTTGAAGA7970.11713060871175436No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC7840.11522007180679475No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC7800.1146322142975764No Hit
GACTTAATAGAAGGGAAGATACACAACAGGTTGCTGGAAACATAGGAAAC7540.1108111404876572No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC7500.11022328297843886No Hit
TAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATA7480.10992935422382968No Hit
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT7360.10816578169617468No Hit
AAGTAATATTTTCCCCCTTCTTAAATAAAAAGCATAAATCCTTAGGAATG7320.10757792418695633No Hit
TAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAAC7260.1066961379231288No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG7130.10478560101816921No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT7120.1046386366408646No Hit
TATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGTT7070.10390381475434168No Hit
ATATTTACCCTTAGTGGAATTCATTGAGCTTCTTAGAGCTATTGATGTGT6980.10258113535860044No Hit
AAATATAACTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTG6850.10067059845364082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)