FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S16_R1_H06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S16_R1_H06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113999
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA473794.253055882455909No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG439893.9487468121605134No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA409553.6763946825805047No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA233692.097757717915366No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA203731.828816722456663No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT172151.5453335236387107No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC156881.4082597919746787No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG144291.2952435325345895No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG136491.2252255163604275No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG129631.1636455688021263No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC123941.1125683236699495No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT120451.0812397497663822No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT119741.0748663149607853No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC114481.0276490373869276No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC107680.966607689953043No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT99520.8933580730323816No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG99030.8889595053496457No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC95060.8533221304507455No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA95020.8529630637011345No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG84710.760413608988877No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC81190.7288157350231015No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC80210.7200185996576298No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA78740.7068228966094224No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG78540.705027562861367No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG74270.6666971873403836No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA70680.6344709465627887No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT69150.6207366433901647No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA67080.6021549390977909No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT64040.5748658661273485No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA60610.5440758923481978No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA58320.5235193209329632No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC56530.5074510838878671No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG55960.5023343827059091No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG55960.5023343827059091No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT54080.48545824547418803No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA50930.45718173894231506No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT49530.44461440270592706No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA49390.44335766908228824No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC49080.44057490177280234No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA48940.4393181681491635No Hit
GCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA48440.43482983377902495No Hit
CCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG47710.42827686559862266No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT46650.4187615967339288No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT43940.3944348244477778No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC43470.39021579013984753No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA43400.3895874233280281No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG43070.38662512264373666No Hit
TCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA42210.37890518752709834No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA42200.37881542083969555No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA41720.3745066198443625No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG41370.37136478578526555No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA39950.358617916174072No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA38580.3463198799998923No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA36890.3311493098288239No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC36840.33070047639181005No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT34630.3108620384757975No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA34300.3078997377915061No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG34030.3054760372316313No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT33050.29667890186615964No Hit
ACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG32810.2945245013684932No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC32410.2909338338723823No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG32310.2900361669983546No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT31970.2869840996266603No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA31570.2833934321305495No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC31470.2824957652565218No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT30740.27594279707611946No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC29930.26867169539649494No Hit
CATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGT29720.26678659496103674No Hit
CAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCA29020.26050292684284276No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC28780.25834852634517624No Hit
CTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTC27970.2510774246655518No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCC27570.2474867571694409No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG27510.2469481570450243No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT26680.23949752199059426No Hit
GGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCAC26660.2393179886157887No Hit
GGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGA26040.23375245399681688No Hit
TAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTC25020.22459625188173418No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG24780.22244185138406766No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT24380.2188511838879568No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG24310.21822281707613742No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTT24000.21544004976665151No Hit
GGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTG23610.2119391489579434No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC23200.20825871477442975No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC23120.20754058127520758No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA22890.20547594746494385No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCA22800.20466804727831892No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT22750.20421921384130506No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC22750.20421921384130506No Hit
GCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTT22360.20071831303259696No Hit
CCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCAT22170.1990127459719443No Hit
GTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTC21660.19443464491440296No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT21070.18913841035763945No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC20920.1877919100465979No Hit
CAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATA20700.1858170429237369No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT20700.1858170429237369No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT20370.18285474223944545No Hit
GCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGA20350.18267520886463992No Hit
AAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC20000.1795333748055429No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC19650.17639154074644592No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAA19540.17540410718501542No Hit
GTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCA19400.1741473735613766No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG18950.17010787262825192No Hit
CTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAA18940.17001810594084915No Hit
CCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAG18640.167325105318766No Hit
GCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTG18430.1654400048833078No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT18150.16292653763603018No Hit
GCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGG18000.16158003732498863No Hit
TCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGC17780.15960517020212764No Hit
GGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAA17780.15960517020212764No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT17700.15888703670290547No Hit
TTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTAT17330.15556566926900295No Hit
TCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCT17270.1550270691445863No Hit
GTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC17200.15439870233276692No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA16650.14946153452561448No Hit
GCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGG16570.1487434010263923No Hit
CTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAAC16260.1459606337169064No Hit
GGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCT16100.14452436671846203No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC15920.14290856634521218No Hit
GAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACC15860.1423699662207955No Hit
GACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAA15570.13976673228611516No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC15300.13734303172624032No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG14890.13366259754272672No Hit
CGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAA14600.13105936360804635No Hit
TTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAA14450.12971286329700474No Hit
CAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCAT14370.1289947297977826No Hit
TATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCA14050.1261221958008939No Hit
TTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTC13820.12405756199063014No Hit
CTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGG13770.12360872855361629No Hit
GATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAAA13720.12315989511660244No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAA13690.12289059505439413No Hit
AATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATC13630.1223519949299775No Hit
CAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGA13600.1220826948677692No Hit
GTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGT13440.12064642786932483No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTC13360.11992829437010268No Hit
TTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCT13290.11929992755828328No Hit
ATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAG13020.11687622699840844No Hit
GTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATA12720.11418322637632529No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCT12690.11391392631411698No Hit
AAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATC12540.1125674260030754No Hit
GTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTT12450.11175952581645046No Hit
GCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGA12370.1110413923172283No Hit
ATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCC12130.10888699181956177No Hit
CTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCC12100.10861769175735346No Hit
CTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCT12090.1085279250699507No Hit
TGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGT11790.10583492444786755No Hit
GAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAA11780.10574515776046478No Hit
TGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGG11730.10529632432345092No Hit
GCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAG11600.10412935738721488No Hit
ACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACA11520.10341122388799272No Hit
GCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTA11140.1000000897666874No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)