FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S16_R1_G07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S16_R1_G07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470054
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA290356.176949882353942No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA207284.409706118871449No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG189774.03719572644845No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA146453.115599484314568No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA96092.0442332157581897No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT79271.6864019878567142No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG68691.4613214651933608No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGC66191.4081360864921904No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC63361.3479302378024651No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC62861.3372931620622313No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG58501.2445378616073899No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG56741.2070953550017658No Hit
GCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGA56431.2005003680428206No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC56411.2000748850132112No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT53421.1364651720866112No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG53361.135188722997783No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT50271.0694515949231365No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT47851.0179681483404035No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC45260.9628680960059908No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG42980.9143630306305233No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA38920.8279899756198225No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG37500.7977806805175576No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG37280.7931003671918546No Hit
GTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTT35970.7652312287524412No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC35460.7543814114974025No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT34970.743957077271973No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC34490.7337454845613484No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA34220.7280014636616219No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA33410.7107694009624426No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA31170.6631153016461938No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT30650.6520527428763504No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA30450.6477979125802568No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA28310.6022712284120548No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG26730.568658069072915No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG26610.5661051708952589No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA26140.5561063196994388No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC25470.541852638207525No Hit
CCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG24630.5239823509639318No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA24560.522493160360299No Hit
TCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA22500.4786684083105346No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC22430.4771792177069018No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA22130.47079697226276135No Hit
GCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACT21510.457606998344871No Hit
ATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATC20870.4439915413973714No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT20710.44058767716049646No Hit
ATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCAT20680.4399494526160824No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA20590.43803477898284027No Hit
GCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA20040.42633399566858277No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC19740.4199517502244423No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG19690.41888804265041885No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA19620.4173988520467862No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA19520.4152714368987393No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGT19430.41335676326549714No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG19230.40910193296940356No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA18970.4035706535844818No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC18440.3922953532998336No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT17790.3784671548375293No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT17510.3725103924229982No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCC17250.36697911303807645No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCA16890.35932041850510793No Hit
CATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGT16360.34804511822045975No Hit
ACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG16110.3427265803503427No Hit
CAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGA16110.3427265803503427No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT15600.33187676309530395No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC15190.32315436098831196No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGG14830.31549566645534344No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG14420.3067732643483515No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAAC14120.300391018904211No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT13890.2954979640637033No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA13770.29294506588604713No Hit
GGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCG13270.28230799014581304No Hit
GGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGA13250.28188250711620366No Hit
GGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTG12770.27167091440557894No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG12680.2697562407723368No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTT12660.2693307577427274No Hit
CTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTC12590.2678415671390947No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC12390.263586736843001No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG11970.2546515932212044No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT11900.25316240261757156No Hit
GTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCA11810.25124772898432945No Hit
CAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCA11780.2506095044399154No Hit
GGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCAC11690.2486948308066733No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC11510.24486548354018897No Hit
CCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCAT11220.2386959796108532No Hit
CCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCC11040.23486663234436894No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT10970.23337744174073616No Hit
GTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTC10940.23273921719632212No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC10900.2318882511371034No Hit
GCTCAGTTCATTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTG10640.22635697175218167No Hit
CAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATA10410.22146391691167397No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT10400.22125117539686928No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT10330.21976198479323655No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT10250.21806005267479908No Hit
GCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTT10090.21465618843792417No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCA9740.20721023541976027No Hit
CAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGC9720.20678475239015093No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT9710.20657201087534624No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT9510.20231718057925258No Hit
CCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAG9460.2012534730052292No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAA9430.20061524846081516No Hit
TAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTC9300.19784960876835428No Hit
GAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAA9210.19593493513511215No Hit
TCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGC9170.1950839690758934No Hit
GCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGA8840.18806349908733888No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC8720.18551060090968272No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG8680.184659634850464No Hit
GAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACC8400.1787028724359329No Hit
TTGATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAA8380.1782773894063235No Hit
TCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGC8320.17700094031749541No Hit
TATTTCCGTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCA8170.17380981759542521No Hit
GCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGG8100.17232062699179243No Hit
TTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTAT8070.1716824024473784No Hit
GACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAA8000.1701932118437456No Hit
CTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAA7920.16849127972530814No Hit
AAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC7790.1657256400328473No Hit
GGATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAA7620.16210903428116769No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCT7580.16125806822194896No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC7490.15934339458870683No Hit
CTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCC7370.15679049641105064No Hit
GCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGG7220.1535993736889804No Hit
TCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCT7040.14977002642249615No Hit
GTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGT6880.14636616218562123No Hit
TTTCATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCT6650.14147310734511354No Hit
GGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCT6640.14126036583030885No Hit
GACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATCAATGAACTGA6530.13892020916745734No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTC6520.13870746765265268No Hit
CTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAAC6500.1382819846230433No Hit
TTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTC6410.13636731098980115No Hit
GAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAA6380.13572908644538711No Hit
GTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTC6340.1348781203861684No Hit
CTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGG6270.1333889297825356No Hit
CGGAAATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAA6260.13317618826773095No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG6200.13189973917890285No Hit
CAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGA6090.12955958251605135No Hit
AAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATC6010.12785765039761388No Hit
GACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAA5870.12487926919034834No Hit
GCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAG5800.12339007858671557No Hit
GTTATCCTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTT5790.12317733707191089No Hit
ATTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCC5700.12126266343866876No Hit
GATGATGGCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAAA5670.1206244388942547No Hit
CGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAGAA5660.12041169737945003No Hit
CTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAA5650.12019895586464534No Hit
GTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATA5620.11956073132023129No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC5590.11892250677581724No Hit
GATGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATT5560.1182842822314032No Hit
AATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCATC5460.11615686708335637No Hit
GCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGA5380.11445493496491893No Hit
CTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCAT5310.11296574436128616No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT5300.11275300284648147No Hit
CTCTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCT5300.11275300284648147No Hit
CAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAGCAT5280.1123275198168721No Hit
TGTCTGCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGT5270.11211477830206742No Hit
TGCTTAGTGCTGGCCCATATTTCCGTTATCCTCTTGTCTGCTGTAATTGG5170.1099873631540206No Hit
GCAATGAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGC5090.10828543103558314No Hit
ACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCA5060.1076472064911691No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCA4980.10594527437273166No Hit
ATCCAATTACAGCAGACAAGAGGATAACGGAAATATGGGCCAGCACTAAG4960.10551979134312227No Hit
CTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCCAAT4950.1053070498283176No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG4910.10445608376909887No Hit
GTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAA4890.1040306007394895No Hit
ACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACA4840.1029668931654661No Hit
GAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATG4800.10211592710624737No Hit
GCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTA4740.10083947801741927No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)