FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S16_R1_G03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S16_R1_G03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819655
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAGAGTGTGGGTTTTAGAGTTAGTGAGATCTCAATCCAATCCTGTC19200.23424489571832052No Hit
GTACAGTAAAGTGGCTCACCCTACAGCTGCCTCGGGAAGTGGAGATGGTG19090.23290286766993432No Hit
CTCTTAATGTATCACCCAAGTTGGGTTCAAAAATAAGGAAGATGCAACTC18080.22058061013475183No Hit
TAATTATACACTGCAACCCAATTCTATGACCTGATCCTTTCTTTTTCATA16900.20618430925206338No Hit
GTCCAGGATATTTAAAATGCAGGGAATGACCAAATGTGTTTAAACGCTGT16230.19801013841189283No Hit
GATAAATACTGTCTTTACGAAGTCTATGTTTCTTCATCTGTAAAATGGGG16120.1966681103635066No Hit
TGATAATACCTCCTTGGAATTGTTAGGAGGATAAAGAGAGATAGTAAAAT15960.19471606956585394No Hit
CCCTACAGCTGCCTCGGGAAGTGGAGATGGTGGGGCAATGTGGCTGGTGC15770.19239802111864138No Hit
ATTATACACTGCAACCCAATTCTATGACCTGATCCTTTCTTTTTCATATG15290.18654189872568339No Hit
GTGTTAGCCATCTTTATTGTCATAATTATACACTGCAACCCAATTCTATG14430.17604967943830027No Hit
ATCTTACACTAGGACAGATACCTGTTTTCAAGGAAATCTTCTAGTTTGGG14010.17092557234446198No Hit
CTATAGAATACTTCATATATGCGAGCTCTTAATGTATCACCCAAGTTGGG13470.16433743465238423No Hit
GTATAATTATGACAATAAAGATGGCTAACACAATGCAAAAACCTTTATTT13080.15957933520810583No Hit
GGATAAAGAAAAGGATACGCAAAAGAAGCGAACGGAAATTTCAGAGATAA12790.1560412612623604No Hit
CCTTATATTTGAGATATGGATAATGATAATACCTCCTTGGAATTGTTAGG12350.15067314906881554No Hit
ATATAATATCTCTCACATGCCTAGCACCCAGCAGGTTCTTAGGATTTGAA12190.1487211082711629No Hit
ATCCTGGACCATCCCCAGGATACCAGTGCACTGCTAAGCCTCATGGGAGA12140.1481110955218964No Hit
GTTTAAAAACTATAGAATACTTCATATATGCGAGCTCTTAATGTATCACC10940.1334707895395014No Hit
GTATTAAATGGTAGCCTAACTGGCCTGTTGACTCTTTTTGTCTTTACATC10310.12578462889874398No Hit
GGCATATAATATCTCTCACATGCCTAGCACCCAGCAGGTTCTTAGGATTT10240.12493061104977095No Hit
GTGTATAATTATGACAATAAAGATGGCTAACACAATGCAAAAACCTTTAT10100.12322257535182485No Hit
CATTAAGAGCTCGCATATATGAAGTATTCTATAGTTTTTAAACAGCGTTT10080.12297857025211827No Hit
GGTAATAAATGCTACTACCATTGAGTTAAGTCCAGAATGTTATGAGAGCC9880.12053851925505243No Hit
TCTTTATCCAGTGTGTTGAAGTTCTACCTCAGACCCTCACGAACATTTGT9850.12017251160549255No Hit
TTATAACACAGGTAATAAATGCTACTACCATTGAGTTAAGTCCAGAATGT9840.12005050905563926No Hit
GATAAAGAGAGATAGTAAAATAAAGGTTTTTGCATTGTGTTAGCCATCTT9620.11736645295886683No Hit
ATATATGCGAGCTCTTAATGTATCACCCAAGTTGGGTTCAAAAATAAGGA9520.11614642746033393No Hit
GCCTAGCACCCAGCAGGTTCTTAGGATTTGAAGAAGAGGCAACTATAAAC9430.1150484045116543No Hit
ATTTAATACACACAGAATTTTTAGAGTAGATATTGGAAGATTAGATGAGA9400.11468239686209443No Hit
GGCTAACACAATGCAAAAACCTTTATTTTACTATCTCTCTTTATCCTCCT9280.11321836626385492No Hit
TCATATATGCGAGCTCTTAATGTATCACCCAAGTTGGGTTCAAAAATAAG9140.11151033056590882No Hit
GTATTTATCTGGTGGGTGGTAGATTTGAATGTATGTGGTATGGTTCCAAA9110.11114432291634896No Hit
CTCTTTATCCTCCTAACAATTCCAAGGAGGTATTATCATTATCCATATCT8990.10968029231810945No Hit
TCTATGACCTGATCCTTTCTTTTTCATATGGCCAATTTTGTTGAGTTGCA8590.10480019032397776No Hit
CTTCATATATGCGAGCTCTTAATGTATCACCCAAGTTGGGTTCAAAAATA8470.10333615972573827No Hit
ACTCCATAAGGTGAAATGATACCAAAATTAGAAAGAGTACAGGATTTGGA8360.10199413167735205No Hit
GTATTGCAGGAATGGAAGAATGTGACTGGAATGGTAGATTGGGTCCTGGT8340.10175012657764547No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)