FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S16_R1_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S16_R1_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560633
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCA16960.30251519264831006No Hit
ATATAGTACAGGCACCATAGTCCTCTTCTAAAATCCAGCAAATTCCAAAT15220.2714788462327405No Hit
ATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAAC13820.24650707325469604No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG10460.18657481810738932No Hit
TTATTAGGGTGACTTCCCGCTAAGATTTAGTTCTTCTTTAAATGGTTTCT9800.17480241084631123No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC9270.16534881107605154No Hit
GTCTTAATGCATCAACATTCCTGCAGTCAAATGTTTGAATCCTTGTATCA9260.1651704412690655No Hit
GTTGTTATTAGGGTGACTTCCCGCTAAGATTTAGTTCTTCTTTAAATGGT9050.16142467532235882No Hit
TCCTAATACTGTAACACTTGAAGAGATGGATCACCATTAGTAATCTTTAC8200.14626324172854613No Hit
TCATAAGACAGAGCAACCTACTGTCCTCTATCCACAATTCCAAATTTTAA8040.14340932481676963No Hit
GTCTTATGAGCCCCATTTTATTAGTGAGGAAACCCAGGTACAGAGCATAT7810.13930681925609087No Hit
CTATTAAGTCTGTTTTCACATTCGCCGCCTTCTCCTTTCCCAAGAGAAGG7800.13912844944910485No Hit
GTACTATATAGTACAGGCACCATAGTCCTCTTCTAAAATCCAGCAAATTC7600.13556105330938423No Hit
GTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAACATTCC7590.13538268350239818No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT7460.13306387601157976No Hit
GTCCAATGTGGGTCTTGATCCCAGGATCCTGAGATCATGACCTGAGTTGA6880.12271842720638992No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC6680.11915103106666929No Hit
GATATGAACAATATTTGGTTCTAAAATGTGACTTGTCTTTAATTGGGAAA6560.11701059338283691No Hit
ATTAAATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGC6550.11683222357585087No Hit
TAGATATGCTCTGTACCTGGGTTTCCTCACTAATAAAATGGGGCTCATAA6400.1141566764710604No Hit
CAATAAAGCAGGGAAAGAGGAATGAATCTTTGGGGTATGTGGTTGTGCAT6350.11326482743613023No Hit
GTACTACCACCGGTACCTCCAGGAAGTCATCAACGTGCTGGAGACAGACG6340.1130864576291442No Hit
ATTCTGGACAGAGGGACACACGGCGTTTGTGAAGGCCCCACGCAGCACAG6320.11272971801517215No Hit
GTGTTGAGAAGTAACAATAAAGCAGGGAAAGAGGAATGAATCTTTGGGGT6300.11237297840120007No Hit
AAAGCAGGGAGTCCAATGTGGGTCTTGATCCCAGGATCCTGAGATCATGA6140.10951906148942356No Hit
CTTTATAGGTTTCTCTAAATGTTGAACTTCAGGTCAAATTGGGTGAGAAC6130.10934069168243753No Hit
GGGTAGGAGAACTAAGTGATTGTTGCCAAGATTTGTCATTACATTAGTGA5990.10684351438463309No Hit
GTATGAAAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACGGTGG5960.106308404963675No Hit
ATACCATACGGAGCACAGGATGAGATTTGAACTTGGGCAGTCTGCTCCAG5920.10559492573573087No Hit
GTATGAAGAAGGTCTTGGGGTTAAACCTGTTGGGGTCCAATCCATCCAGC5780.1030977484379264No Hit
TATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTGAA5750.10256263901696833No Hit
GTTGTGTATCTTCCCTTCTATTAAGTCTGTTTTCACATTCGCCGCCTTCT5650.10077894094710799No Hit
ACATTAGTGACAGTGTTGAGAAGTAACAATAAAGCAGGGAAAGAGGAATG5610.10006546171916386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)