FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S16_R1_C12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S16_R1_C12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947799
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCA21750.229479035111875No Hit
ATGTAACACAATGCTTGACTTCAGGGGAAATACTCAACCAAAAAATCTAG15590.16448635206409798No Hit
ATGTAACACAATGTAACACAATGTAACACAATGCTTGACTTCAGGGGAAA14990.15815589592308074No Hit
GTGTTACATTGTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAG14480.15277500820321607No Hit
TCCCCGAAGTAACTCATTCACCCGTTAAATAAATAGTGATCCAACATCAA13030.13747640586242443No Hit
GTGTTACATTGGGCCTTACCTCATCTGGTCTTATCAGCAGGGCTGTGAAA11710.12354940235218649No Hit
TCTTATATGTTCTTGATTTTAAGACAAAGGCTGCATGATTTGAGCTAATG11380.120067651474627No Hit
TATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACCTG11090.11700793100646867No Hit
CTCCCTAAAGGGACAAGATGTTATAAAAAGAAAGAAATACATTGACAGCG11010.11616387018766637No Hit
GATATATATAGAGAGAGATGGATCGATCAAAACACATCAAGTTATCCACC10660.11247110410540631No Hit
CCACAGGGCCAGTCCCTGAGTCTGTCACATACACAGCAATACAAGCTTCT10500.11078298246780172No Hit
CTTTAGCGAGTCTCATCGGCTGCAAATCCATAAAACACCCTGCACTTCCA10270.1083563076137451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)