FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S16_R1_B05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S16_R1_B05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886635
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG29290.33035014408409324No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA28830.3251619888680235No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA23950.27012242918450097No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT23870.26922014132083666No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22980.25918218883757127No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA22370.25230224387713096No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA19600.22106052659775444No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC18760.21158650402927923No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT17770.20042069171643345No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16840.18993159530133596No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT16310.18395393820455994No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG16270.18350279427272778No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT16250.1832772223068117No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA15780.17797628110778393No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15620.17617170538045532No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA15540.175269417516791No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14360.16196067152774254No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13920.15699808827758885No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA13630.15372729477180574No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA12840.14481720211812077No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC12820.1445916301522047No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC12640.14256148245896No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC12610.14222312451008587No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT12180.13737332724289025No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT12140.1369221833110581No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC12050.13590710946443577No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG11830.13342581783935892No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11600.13083174023132405No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11520.12992945236765974No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT11440.12902716450399543No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC11220.1265458728789186No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11220.1265458728789186No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA11190.1262075149300445No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA10840.12226000552651316No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA10260.11571841851494696No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC10130.11425220073649246No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT10070.11357548483874425No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT9740.109853547401129No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG9340.10534210808280746No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA9310.10500375013393336No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG9180.10353753235547887No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA9050.10207131457702437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)