FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACGTAACTATTTCGACACTGTCACATTTGCGCGGACACCAGAGT27.4074074074074066No Hit
TCTTGGCACAGGCCCAGCCAACAAGGAGAAGAACGCGTGAAGATCACCGA13.7037037037037033No Hit
ACTCTCTCTTCTATGCATCTTTTATGACCTTCTATGATGCTTATTTATGG13.7037037037037033No Hit
ATATTTTCACCGAGTGACCACTTCAATTGGCTTGTTCTCTTCATGTTCTT13.7037037037037033No Hit
ATACGTAACTATGCGGCCGGAATGCCGCCATCCCTGGCGGCGTTCCCAGA13.7037037037037033No Hit
ATTGTGACCCGTGCGACGCACTCCTGGATTGGGAAATGTGACATGGTCGA13.7037037037037033No Hit
GTGGCCTGCCATCCACACCAATTGACTGTTGTGAGGTGGGTTTTGATTTG13.7037037037037033No Hit
ACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAAATGAT13.7037037037037033No Hit
ACATTTCCCAATCCAGGAGTGGGGGGGCCCGGGGGCCATCGGTTTTTTGC13.7037037037037033No Hit
TCGCACGGGTCAGGAAGCAGTTCCAAGTGCGAATAACGATTGTGACCCGT13.7037037037037033No Hit
GAATTGACGAATCGTACAACCTGAATAAAATGTCTATACGTAACTATGCG13.7037037037037033No Hit
GATTGGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCCGCA13.7037037037037033No Hit
ACGCACTCCTGGATTGGGAAATGTGACATGGTCGACCGATTCAGGCGTCA13.7037037037037033No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT13.7037037037037033No Hit
TCTCAAGGGAGAAGTCCGGAACAACAGAAGTTTTAGGCTTCGTGAACAAA13.7037037037037033No Hit
CAGCCAAGGGTACGCCACACTCCCAAGGCTGCCCAGTATACAGGTACTCG13.7037037037037033No Hit
GGGAAATGTGACATGGTCGACCGATTCAGGCGTCAGGTATCACCTCTGGG13.7037037037037033No Hit
CCTCTGGGAACGCCGCCAGGGATGGCGGCATTCCGGCCGCATAGTTACGT13.7037037037037033No Hit
CACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTC13.7037037037037033No Hit
CTCCTGGATTGGGAAATGTGACATGGTCGACCGATTCAGGCGTCAGGTAT13.7037037037037033No Hit
AAGCAGGGGTGGGTCGATGATGGCAATGGGGGTCACGTGCATGTCGGTGA13.7037037037037033No Hit
CAACCGGATCGTGGCTTCCTTCAGCGAAGATGTTGTCGAACTGGGAGCCG13.7037037037037033No Hit
CTGTCACATTTGCGCGGACACCAGAGTGGGGGGGCCCGGGGGGGGGGGCG13.7037037037037033No Hit
GAGCCCTGTTGCTTGAATTGACGAATCGTCCCACCTGAATAAAATGTCTA13.7037037037037033No Hit
TTATCATACTTCCTCAGCATAACCACAACCTTGGTTAAATTCAACTTTCT13.7037037037037033No Hit
AAGCAATGGCGTCTCAAGGCACCAAACGATCATATGAAAAAATGGAGACT13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)