FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_G12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_G12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19939
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATGGGCGTGTCTGTGATCGAAGCGCTGATAGCGCTGGCCACGCTGGG760.381162545764582No Hit
GTATTATAAGACGCCCGGACAACTTCGGCTACTGTCGCTGCAAATAATTT730.3661166558001906No Hit
ATATAAGGGCGTCTATAAGAGCCTTCCTAAATCCTTATATATATTGAAAA690.34605546918100205No Hit
GTCAAGAAGGGCATCACCTTCTTGGCCCAGCTTTTGTTGCGCCTGCGCAT690.34605546918100205No Hit
GTCTTTACACCGAATGCATGTTATTTGGCGATTACGACCATAATCAAAGC620.31094839259742213No Hit
CCCTATGTCCTCCCTGCCTGTTGCCGCAGCAACATCGGTATTCTGGCAAG600.30091779928782786No Hit
GTCATATCCCAGCGACAGGTCTTTTTTACCTTTCGCCAAGATTTTCTGCA600.30091779928782786No Hit
CGCTGGGGGTGTTGTTGGCGCTGTCACTGCCAACGTTTCGGGGCTGGCAG570.2858719093234365No Hit
TTATAATACCGATCATCTAGTTATAGCGACGTACACTTTCATGCCATCTG550.2758413160138422No Hit
GGCGTGGGCTTTGCGTGCCGTTGGGGCTGGATGGTCGCCAAGATCCCGGC530.265810722704248No Hit
GCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACG530.265810722704248No Hit
CCCTTATATAGTCTTAAAAACTGGGGTTTTTGCCGGGAATACCGGGAAAG520.26079542604945083No Hit
GATACGCCCCATGCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTG480.2407342394302623No Hit
GTTCTAACACGGCCTGCGCCTGCGAGATAAGCGCGGCAAGGTCGGGAGCG470.23571894277546515No Hit
AGATTGGAGCGTACAGTCGCAGCCCCCAGCCATGTGCCAGCCGTAGCACC470.23571894277546515No Hit
GTATACGCGGGCCTGCTCATCCATCCAAAGCACTACGCTTTGCAGGGCAA460.23070364612066802No Hit
GGCTACAGCCAGCGCAACGGCAGTCTTCAGCACGGGGGCTCCGGGAAAGA440.2206730528110738No Hit
CCGTAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGGTCAGTCC440.2206730528110738No Hit
TAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGGTCAGTCCGGT440.2206730528110738No Hit
GCTTTTGTTGCGCCTGCGCATGGCGTGGGCTTTGCGTGCCGTTGGGGCTG430.21565775615627666No Hit
ACTTTAGAGAGGCGGCTGTAGATGCCATCCTTAAGAGCAAGATCAGCGTA410.2056271628466824No Hit
GGTTACGACGCGCGGCGCGCCATCGATGTGGCACCCAGATTGGAGCGTAC400.20061186619188526No Hit
GACCCGTACCGAGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGG400.20061186619188526No Hit
GCTCTAGTCTCTGCGGCCATCTCTAAATTCCCTTGTCTAGTTAGTTTAAG390.19559656953708812No Hit
GGGTCAAGAAGGGCATCACCTTCTTGGCCCAGCTTTTGTTGCGCCTGCGC390.19559656953708812No Hit
AAACCGGACTGACCGTCCGTTTCCAGCGCGCCGGCCCGGGGCGGTGGTGC380.190581272882291No Hit
CCCCGAAACAGGGGATGGCGAGCCCATGACCGCCTTGCCAGAATACCGAT380.190581272882291No Hit
ACGTTGGGCTGCAGGTCGATATCCGGCCAATCGTCGCTGGTTACGACGCG380.190581272882291No Hit
GGATAATGAAGAGCCGATGCAGGCCCAAGACCTCACCGAAGGGCTGCAAG370.18556597622749388No Hit
CTATTGCCTCTTTCATGAACGTTCCTTTGATCGATGATCACGAGATGCTT370.18556597622749388No Hit
GCAGGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACGCACGAGC370.18556597622749388No Hit
GCTCATACCTTTCCCCTTACAAAGTCAGTTGGACGATGGTCAGACCTGCA360.18055067957269674No Hit
ACCTCATACGGTTGACCTTCCCAAACGCCGGGTTTTTCTTCGAAGTCGCA360.18055067957269674No Hit
GCCGTACCCCATTCCCTATGTCCTCCCTGCCTGTTGCCGCAGCAACATCG360.18055067957269674No Hit
GTCCGGTTTGGCGCTTGCGTGCCTCCGCCTGCGCAAGGCGCAAATCGTTG350.1755353829178996No Hit
GATTTACAGTGCCCTGAGTGACGCCCGAATGGAAGCGAACGGGGTTGAGG350.1755353829178996No Hit
GTATTGGATGGTCGTTGTTTTGATCATCTCAGTTTTGATTTCACACCATC350.1755353829178996No Hit
GACTAGAGCAGTTCGAAGGGGGCATATTTGTAATTCATTAGCGTCATTCA340.17052008626310247No Hit
AGCGAAAGCAGGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACG340.17052008626310247No Hit
GTATTGGTCGATGTCTGGAGACGACGGAACAGCCGCATCGGCTGCGCGAG340.17052008626310247No Hit
CTCTAAAGTTGTTGGGGCCTTTGATCACAAAGCTATGGACGCACGTGGCG340.17052008626310247No Hit
GGATATGACTGCGACTTCGAAGAAAAACCCGGCGTTTGGGAAGGTCAACC340.17052008626310247No Hit
ATCATAGACCCTTATGAATGACGCTAATGAATTACAAATATGCCCCCTTC340.17052008626310247No Hit
CCATCGATGTGGCACCCAGATTGGAGCGTACAGTCGCAGCCCCCAGCCAT330.16550478960830534No Hit
GCCCCATGCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTGACTGC330.16550478960830534No Hit
GCTATGGACGCACGTGGCGTTATTCGCTACGGGGTGAAAAAGCTGGGTAT330.16550478960830534No Hit
ATCCAGCTGGGCGTGGCTTGAAATGAATTTTTCAGGGGCGACAACTTCGG330.16550478960830534No Hit
GCCCATGACCGCCTTGCCAGAATACCGATGTTGCTGCGGCAACAGGCAGG320.1604894929535082No Hit
CTCTTCCGCTCCCCGAAACAGGGGATGGCGAGCCCATGACCGCCTTGCCA320.1604894929535082No Hit
ATCCAATGCGACTGGTAAACGTGTACCGCCCGGAATCGGCAGTTAGCGAC320.1604894929535082No Hit
GTCTACAGCAGCTGGGTCTGTGATCGGGTCCGCCGATACAGGGTCTTCGC320.1604894929535082No Hit
GTTATATCCCGAACAACGTCGACTCGACCAAAGAGACTACCGGAATCCCG320.1604894929535082No Hit
ATCCAATACTCTTGCGCCCGGCACGCGACCCTCACGCACTAGTGCGATGT310.15547419629871106No Hit
GTGCTACGGCTGGCACATGGCTGGGGGCTGCGACTGTACGCTCCAATCTG310.15547419629871106No Hit
GTCACTGCCAACGTTTCGGGGCTGGCAGGAGGTCCATCGATTGCGCTCGG310.15547419629871106No Hit
CCCTAGCACGGCGAGCGATCCGACACAAGATGAAGGCCCGACAACGGCTC310.15547419629871106No Hit
GGCAGGAGGTCCATCGATTGCGCTCGGCGTCCACCTTGGTGCACAACGAT310.15547419629871106No Hit
TGTTAGAACAGCTGGCGTCGGCAGCAGGCGCCCAAGGACAGGATAATGAA310.15547419629871106No Hit
CGCGTATACTGGGCCACCGAGGGCAACGGTAGGGGCCAGGGCGGCGAGGG310.15547419629871106No Hit
GTCATGGGCTCGCCATCCCCTGTTTCGGGGAGCGGAAGAGCGCTCCGAAG300.15045889964391393No Hit
TAATAAACGTTCCCGGTCAAAACGCCGTCCAAGCCTTTGTCTTCTGGCGC300.15045889964391393No Hit
GCCTCCGCCTGCGCAAGGCGCAAATCGTTGTGCACCAAGGTGGACGCCGA300.15045889964391393No Hit
GGATATAACCGACAAAGGATGCAGGGGTAACGGCCTGCACTGCCAGGGTG290.1454436029891168No Hit
GTACCGAGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGGTGATG290.1454436029891168No Hit
GGCGGAGGCACGCAAGCGCCAAACCGGACTGACCGTCCGTTTCCAGCGCG290.1454436029891168No Hit
ATCACAGACACGCCCATGGCTGGGCGTGGCGCACCGCCGTACCCCATTCC290.1454436029891168No Hit
CCTTGGTGCACAACGATTTGCGCCTTGCGCAGGCGGAGGCACGCAAGCGC290.1454436029891168No Hit
GATAGCGCTGGCCACGCTGGGGGTGTTGTTGGCGCTGTCACTGCCAACGT290.1454436029891168No Hit
AAAGCAGGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACGCACG290.1454436029891168No Hit
GTATCGACTTCGTCACCGTCGAAGCCCGAAAGCATCTCGTGATCATCGAT290.1454436029891168No Hit
ATCAAGTGCAGCCCATTTAAAGCTGAAGAAACCGGGTCTATCATAGACCC280.14042830633431966No Hit
ATATAAGGATTTAGGAAGGCTCTTATAGACGCCCTTATATAGTCTTAAAA280.14042830633431966No Hit
CCGTTGGGGCTGGATGGTCGCCAAGATCCCGGCCGATGGGAACGCAACCC280.14042830633431966No Hit
CTCCAATCTGGGTGCCACATCGATGGCGCGCCGCGCGTCGTAACCAGCGA280.14042830633431966No Hit
CCTTCTTGGCCCAGCTTTTGTTGCGCCTGCGCATGGCGTGGGCTTTGCGT280.14042830633431966No Hit
GCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTGACTGCAGCGTGA270.13541300967952255No Hit
AGCGGATACGCCCCATGCCATGCACGACGACCCGTACCGAGTATCCGCTG270.13541300967952255No Hit
AGTTATAGCGACGTACACTTTCATGCCATCTGCTCGCCATATCGACGCCA270.13541300967952255No Hit
GATTTGCGCCTTGCGCAGGCGGAGGCACGCAAGCGCCAAACCGGACTGAC270.13541300967952255No Hit
GGTCGATATCCGGCCAATCGTCGCTGGTTACGACGCGCGGCGCGCCATCG270.13541300967952255No Hit
GTTGTTAATCGCAACTTCACCACTGGCTTTCCCGGCGAAATTGTTCGTGA270.13541300967952255No Hit
AGTTAAGACCATGGACAAGGCGACGCGTGATAGCGCCGTTGAATCTCTTC270.13541300967952255No Hit
GACCTGCAACCGGCGCACCAACAGCGGACGCGGACAGGCTGATCGAGTTC270.13541300967952255No Hit
AGCAGGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACGCACGAG270.13541300967952255No Hit
GGATTACCTCATACGGTTGACCTTCCCAAACGCCGGGTTTTTCTTCGAAG270.13541300967952255No Hit
GGTAAAGGGCTGCCCTTTATCATCTTTTGGCATCTTTGTTTATTCGGCAG270.13541300967952255No Hit
GTTCATGTCCGAAGAGGCCAAAGAGCCAGAACACCAGGCTGATCCGTCCG270.13541300967952255No Hit
GTGTAGCCGTAAACGCGACCAATCAGGTTGCCGGAGAAACGGGCGTTGGC260.13039771302472541No Hit
CCGATAAGGTTATCCCAAGAAAATCCCGAACCCTGCGGCCCGCCTATCTC260.13039771302472541No Hit
ATTTCAAACTGTTGTTAATCGCAACTTCACCACTGGCTTTCCCGGCGAAA260.13039771302472541No Hit
CTTTCATGCCATCTGCTCGCCATATCGACGCCAACGGTTTTCTTCTGGTA260.13039771302472541No Hit
GGTGTACCGCCTGCGCATCACGTCCCTCGGCAAGCTGATCCTGACCAAGT260.13039771302472541No Hit
GCCCCCAGCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCT260.13039771302472541No Hit
GTATTCTGGCAAGGCGGTCATGGGCTCGCCATCCCCTGTTTCGGGGAGCG260.13039771302472541No Hit
CGTTTATTATGAGAAGCCGTGGATGCGCGGCGACCTCAAAGTGTTTTCCC260.13039771302472541No Hit
CATATTGACTGGCGCAAATTATTTGCAGCGACAGTAGCCGAAGTTGTCCG250.12538241636992828No Hit
GGTGGACGCCGAGCGCAATCGATGGACCTCCTGCCAGCCCCGAAACGTTG250.12538241636992828No Hit
GTCTGTGATCGAAGCGCTGATAGCGCTGGCCACGCTGGGGGTGTTGTTGG250.12538241636992828No Hit
GCGTAGTGCTTTGGATGGATGAGCAGGCCCGCGTATACTGGGCCACCGAG250.12538241636992828No Hit
AAACAGGGGATGGCGAGCCCATGACCGCCTTGCCAGAATACCGATGTTGC250.12538241636992828No Hit
GTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCTGGAAACGGACGG250.12538241636992828No Hit
CAGGTGTACCGCCTGCGCATCACGTCCCTCGGCAAGCTGATCCTGACCAA250.12538241636992828No Hit
GTCGCCAAGATCCCGGCCGATGGGAACGCAACCCGCTTCAATCCAGCTGG250.12538241636992828No Hit
GTGTACCGCCTGCGCATCACGTCCCTCGGCAAGCTGATCCTGACCAAGTA250.12538241636992828No Hit
CCCCCAGCGTGGCCAGCGCTATCAGCGCTTCGATCACAGACACGCCCATG250.12538241636992828No Hit
GTGTAAAGACCTTCCCGGCAAGATGGATCGCCGCTTGCCGGGAAGGTATC250.12538241636992828No Hit
GCGCAGGCGGAGGCACGCAAGCGCCAAACCGGACTGACCGTCCGTTTCCA240.12036711971513114No Hit
ATTAACAACAGTTTGAAATGGCATGGTTATTTGCTCCCCTGCGCGATGTA240.12036711971513114No Hit
GTATGAGGTAATCCAGACCAACTTGCGCGGCAACCACATCGCACTAGTGC240.12036711971513114No Hit
AGCGAAAGCAGGGTCAAGAAGGGCATCACCTTCTTGGCCCAGCTTTTGTT240.12036711971513114No Hit
GGGGATGGCGAGCCCATGACCGCCTTGCCAGAATACCGATGTTGCTGCGG240.12036711971513114No Hit
CCAAGATCCCGGCCGATGGGAACGCAACCCGCTTCAATCCAGCTGGGCGT240.12036711971513114No Hit
ACCATGGACAAGGCGACGCGTGATAGCGCCGTTGAATCTCTTCAAACGCT240.12036711971513114No Hit
GACATGAACTGTTCAAGCGCTTGGGTGAGTTGCGGAATCAGCGTTTGAAG240.12036711971513114No Hit
CTTCAGCACGGGGGCTCCGGGAAAGAGCGCGGGCTGGCTGAGGCTGGCTG240.12036711971513114No Hit
GCACTCAGAGCGCTGATGCGGATAGCCTTGGCACGAGTTGGGCCGTCACG240.12036711971513114No Hit
GTCTTAACTTTAACGCCCATGGTGTTCTCCTTATCGGATGGTGTGAAATC230.11535182306033401No Hit
CCCAAGGACAGGATAATGAAGAGCCGATGCAGGCCCAAGACCTCACCGAA230.11535182306033401No Hit
GGCTTGGACGGCGTTTTGACCGGGAACGTTTATTATGAGAAGCCGTGGAT230.11535182306033401No Hit
TCCTTATATATATTGAAAAATCGAATTCTATCCGGGAATACCGATAAGGT230.11535182306033401No Hit
TCCTAAATCCTTATATATATTGAAAAATCGAATTCTATCCGGGAATACCG230.11535182306033401No Hit
ACCATAATCAAAGCGATAATGGCTATATCAAGTGCAGCCCATTTAAAGCT230.11535182306033401No Hit
GTCTATGATAGACCCGGTTTCTTCAGCTTTAAATGGGCTGCACTTGATAT230.11535182306033401No Hit
CCCCAGCCATGTGCCAGCCGTAGCACCACCGCCCCGGGCCGGCGCGCTGG230.11535182306033401No Hit
GGGAGGGATCCCGGCAAGCGGAGGGCTGGCACTCGGGACGCACGAGCGGA220.1103365264055369No Hit
ATCCCGAACAACGTCGACTCGACCAAAGAGACTACCGGAATCCCGTTCGG220.1103365264055369No Hit
GTTATTTGCTCCCCTGCGCGATGTATTGGTCGATGTCTGGAGACGACGGA220.1103365264055369No Hit
GATTGGAGCGTACAGTCGCAGCCCCCAGCCATGTGCCAGCCGTAGCACCA220.1103365264055369No Hit
ATACGGGCCAGACCGGCCACGGCTTGATCGGTTACTTGCTTCATCTCCAG220.1103365264055369No Hit
CGTTAACGCGGCTCATACCTTTCCCCTTACAAAGTCAGTTGGACGATGGT220.1103365264055369No Hit
GGCTTTGCGTGCCGTTGGGGCTGGATGGTCGCCAAGATCCCGGCCGATGG220.1103365264055369No Hit
GCCCATGGCTGGGCGTGGCGCACCGCCGTACCCCATTCCCTATGTCCTCC220.1103365264055369No Hit
GCCCAGCCATGGGCGTGTCTGTGATCGAAGCGCTGATAGCGCTGGCCACG220.1103365264055369No Hit
GGAAACGGACGGTCAGTCCGGTTTGGCGCTTGCGTGCCTCCGCCTGCGCA220.1103365264055369No Hit
CACTAGTGCGATGTGGTTGCCGCGCAAGTTGGTCTGGATTACCTCATACG220.1103365264055369No Hit
ATTATGTTCTGAGTGCAACTAAGCAGACAACTAGCGGCCACATATTGACT210.10532122975073976No Hit
GGCCACGCTGGGGGTGTTGTTGGCGCTGTCACTGCCAACGTTTCGGGGCT210.10532122975073976No Hit
GGGCTGGATGGTCGCCAAGATCCCGGCCGATGGGAACGCAACCCGCTTCA210.10532122975073976No Hit
GTAGGGGCCAGGGCGGCGAGGGTCTGGCCAGTCGCCGGAACTTCGCCGGT210.10532122975073976No Hit
CATCGATGGCGCGCCGCGCGTCGTAACCAGCGACGATTGGCCGGATATCG210.10532122975073976No Hit
GGTCAGACCTGCAACCGGCGCACCAACAGCGGACGCGGACAGGCTGATCG210.10532122975073976No Hit
GTCCAAGCCTTTGTCTTCTGGCGCGGTATCGACTTCGTCACCGTCGAAGC210.10532122975073976No Hit
TTGCAGGGCAACTCGGTTGACGGCCCACTGGGCGCCTCGCTGGCAGTGCC210.10532122975073976No Hit
GCTGTAGACCCTGCTCAGGCCGACCCTGCCAACGCAGACGACCCGAACGC210.10532122975073976No Hit
GCACCAAGGTGGACGCCGAGCGCAATCGATGGACCTCCTGCCAGCCCCGA210.10532122975073976No Hit
AGTATCCGCTGGGTGACTGCAGCGTGACGTTGCCAGCGGTGATGGTTCCG210.10532122975073976No Hit
GGAGAACACCATGGGCGTTAAAGTTAAGACCATGGACAAGGCGACGCGTG210.10532122975073976No Hit
GTCCATGGTCTTAACTTTAACGCCCATGGTGTTCTCCTTATCGGATGGTG210.10532122975073976No Hit
GACCAATACATCGCGCAGGGGAGCAAATAACCATGCCATTTCAAACTGTT210.10532122975073976No Hit
GCCAGAATACCGATGTTGCTGCGGCAACAGGCAGGGAGGACATAGGGAAT200.10030593309594263No Hit
CTTCCGCTCCCCGAAACAGGGGATGGCGAGCCCATGACCGCCTTGCCAGA200.10030593309594263No Hit
TTTCAGGGGCGACAACTTCGGAGCGCTCTTCCGCTCCCCGAAACAGGGGA200.10030593309594263No Hit
CTATCAGCGCTTCGATCACAGACACGCCCATGGCTGGGCGTGGCGCACCG200.10030593309594263No Hit
GCGTCCACCTTGGTGCACAACGATTTGCGCCTTGCGCAGGCGGAGGCACG200.10030593309594263No Hit
ATATCCGGCCAATCGTCGCTGGTTACGACGCGCGGCGCGCCATCGATGTG200.10030593309594263No Hit
CCCCATGCCATGCACGACGACCCGTACCGAGTATCCGCTGGGTGACTGCA200.10030593309594263No Hit
TCTTGGCCCAGCTTTTGTTGCGCCTGCGCATGGCGTGGGCTTTGCGTGCC200.10030593309594263No Hit
GGCTGGCACTCGGGACGCACGAGCGGATACGCCCCATGCCATGCACGACG200.10030593309594263No Hit
ATCCAAAGCACTACGCTTTGCAGGGCAACTCGGTTGACGGCCCACTGGGC200.10030593309594263No Hit
CCTTATCGGATGGTGTGAAATCAAAACTGAGATGATCAAAACAACGACCA200.10030593309594263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)