FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_G01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_G01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162510
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG27730.23853558248961299No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG26060.2241701146656803No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT25500.21935295180256514No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT22290.19174028610506574No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20420.17565440297287765No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT19610.16868672097444323No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG19020.16361149581508974No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA18220.1567298345820681No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC17940.15432125315051054No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT17800.15311696243473175No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT17390.14959011105280814No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC17110.14718152962125058No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG16410.1411600760423566No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA15880.13660097547547978No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14820.1274827743417261No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14640.12593440056429622No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA14450.12430000602145358No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC13480.11595599177641482No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT12990.11174097427118906No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC12930.11122484967871243No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA12830.11036464202458472No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12760.10976249666669535No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC12690.10916035130880594No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12600.10838616442009101No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA12450.10709585293889945No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT12240.10528941686523126No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA12150.10451522997651634No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA12100.10408512614945248No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG12040.10356900155697585No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC11990.103138897729912No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT11970.10296685619908645No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA11870.10210664854495875No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA11730.10090235782917996No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG11720.10081633706376719No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)