FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_F07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_F07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970356
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA23570.24290054371797565No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT22190.22867895906244717No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA20190.20806796680805806No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19070.19652581114560017No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC16840.17354455478195632No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT16550.1705559609050699No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA16400.1690101364859907No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT15270.15736492586226086No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG14740.15190301291484776No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT14690.15138773810848802No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC14460.14901747399923326No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT14390.14829608927032964No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT13990.1441738908194518No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA13780.14200973663274097No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT13530.13943336260094233No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC13290.13696004353041563No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA13200.13603254887896815No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC12780.13170424050554644No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT12740.13129202066045864No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12730.13118896569918667No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC12500.12881870158993194No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT12330.12706676724830887No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA12330.12706676724830887No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG12310.12686065732576496No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA11840.12201707414598353No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA11780.12139874437835185No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA11570.11923459019164101No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT11520.11871931538528128No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG11500.11851320546273737No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC11380.11727654592747405No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA11360.11707043600493014No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC11290.11634905127602653No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA11200.11542155662457902No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT10970.11305129251532428No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT10860.11191768794133287No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT10840.11171157801878899No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA10710.1103718635222537No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT10610.10934131390953424No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG10560.10882603910317451No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG10240.10552828034247225No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG10210.1052191154586564No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG10040.10346718111703335No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA9970.10274579638812971No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT9930.10233357654304194No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT9920.10223052158176998No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT9920.10223052158176998No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA9860.1016121918141383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)