FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_E08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_E08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967624
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTATACCATGAGCATTTTCCCACAATCATGTGCTATCCCTCCCTTAC18640.1926368093391648No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA11420.11802104949856557No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA10660.1101667589890288No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT10250.10592957595098923No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA9910.1024158144072491No Hit
GTTTTAGATTTGGGCATCCTTTTTTCTACTTTATCTTATACCATGAGCAT9850.10179573884070672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)