FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_A05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_A05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTATGATATGCTGA13.7037037037037033No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC13.7037037037037033No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC13.7037037037037033No Hit
GGAGTATATGTGAGAAACTTGAACAATCAGTGTTGACAGTTTGAGGCAAT13.7037037037037033No Hit
CCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAATG13.7037037037037033No Hit
GATACTGGTTGAAAGACACCCACGGTCGATTACAGCCATGCCAGTTATCC13.7037037037037033No Hit
GTACGTGTACCTGTAATCTTTGATGAACAAATGAAGGGCCATTTGAGCTG13.7037037037037033No Hit
GATTATAAAGTACTTAACATTTTAGGCCAATTATGCTGGCTGGCAGTACC13.7037037037037033No Hit
GGTTATTAGTATTCGCCGCTCACGGCATGAGGAAGGGGAGGAACTCGTTG13.7037037037037033No Hit
GGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCT13.7037037037037033No Hit
CAATGGAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTC13.7037037037037033No Hit
AAGTTACAGTTCACCAAAGTTTTAAAACATCTGTACATCAAAAAACGCTA13.7037037037037033No Hit
GTCAGTGTGCAAATCAGTGTTCTGGTCTGTTCTCCTTATGCATGTAATTT13.7037037037037033No Hit
GTATACAAACCCCCTTATTTGCATCACTTGGCATGCAATTGCTCTCCGAT13.7037037037037033No Hit
GTGAATACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCA13.7037037037037033No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT13.7037037037037033No Hit
CTTCATTATGTATTCAGTAGCCCTTAAGTCGGACACTTATTATGTAAAAT13.7037037037037033No Hit
TTCCCTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAA13.7037037037037033No Hit
CAAAATAACATAGAACACAAAGAGATCTTCATATAAAACTTTAACTGAGT13.7037037037037033No Hit
ACTGAAGGGCCATTTGAGCTGTTGCTGGACCAAGATCATTGTTTATCATA13.7037037037037033No Hit
GCCTAATACAAATCTTAAAATATAGAACATGAAAATCAACATTACACGTC13.7037037037037033No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT13.7037037037037033No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC13.7037037037037033No Hit
ATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAA13.7037037037037033No Hit
AAAGTATACAAACCCCCTTATTTGCTTCCCTGGGGTTGGAATTGCTCTAC13.7037037037037033No Hit
CTGCAACAGCATTGGCCAACACAATAGAAGTGTTCAGATCAAATGGCCTC13.7037037037037033No Hit
ACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAG13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)