FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_A03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_A03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTCGGTACAACTATATTTCAAGATGATTCGAATGTAATTTTTTCTAC13.125No Hit
GTTCTGCATTCCAAGGGGGAGCATGATATGAATGGTTCCCTTATGACAAA13.125No Hit
AGTATGGATAGGAAATAGTAGAATTGCCACAACTACTTCAGTGCATGTGT13.125No Hit
AAATACGGCAATGGTGTTTGGATAGCGAGAACTAAATGCATTAGTTCAAG13.125No Hit
TGCAGCGAGTGCAAGAAGTTCAAGCCGGAAAGACGAATCAGCCGGGAAGA13.125No Hit
GTTCCATAGGGGAGACACAAAAATTCAGACGAGAAGATCATTTGAGTTAA13.125No Hit
GGTACTCACGTGTATGGATATACCCAGTCAAATTACTACAAAAAAGCCAA13.125No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA13.125No Hit
ACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGT13.125No Hit
CAAGAAGGTCATCTTTCAGACAAGCACTGGATCTAGGATGAGTCCCAATA13.125No Hit
TTATTATGCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCA13.125No Hit
ACCTTGGGAAGATGACCTGAGCCAAAGGCAGACGTTTAACTGACTGAGCC13.125No Hit
GCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGA13.125No Hit
GAGACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCA13.125No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA13.125No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG13.125No Hit
CCTCATATACAACAGGATGGGAACATTGACAACATAAGCTGCTTTTCGAG13.125No Hit
AAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGA13.125No Hit
GCCCTCCATCATGCTACGCCGTGAACCTCTGGAGGATACTGAGTCTGTCG13.125No Hit
CCACATATCAGAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGA13.125No Hit
ATAGCATAGACTCAAGGAAAATATAATTCTTTATTTTATTTATATTTTAG13.125No Hit
CCTAAAAGGCAATAATGCCTTTCTCATGTGATTTGACTATGTAAGAAACA13.125No Hit
CAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAA13.125No Hit
GTCCGAAAGAGGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAA13.125No Hit
GAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCACAAAAGC13.125No Hit
GTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGGAGGG13.125No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG13.125No Hit
CATCAATACATCTATGGAATAGCAACAGAAAACACCATGGACACAGTTAT13.125No Hit
CTTTTCATACTGAGCGAAAGACAGAACCCGCCACGGGCTTAATTGATTGA13.125No Hit
ATCGTGGACTGGTGTATCTGAAATGATAAAACCAGATACAGCATTAATTT13.125No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT13.125No Hit
CCTTAGCGCGGAGGGGGAAAGGCAGGAGAACTATAAAAACGAAAAAGTAT13.125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)