FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S12_R1_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S12_R1_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACCCCACCTCCTGGCAGGCAAATGAGCATCCCTGTGTTTGTGCTGGG12.857142857142857No Hit
CTTAGAATAGTGCGCACCTTATAATTTCCATTTTTCCTTCTCCTACCTTG12.857142857142857No Hit
AACATATACTCTTGACCTCAGACAACAGCTTGAGAATCACACAAGGATCA12.857142857142857No Hit
CATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGATA12.857142857142857No Hit
TCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGGCAATGATGGATCAA12.857142857142857No Hit
ACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAG12.857142857142857No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTTAAGAATCCCACCCAGGAATATTT12.857142857142857No Hit
GTGCAGATGCAGAGATTAAATTTATCCTAGCTTCATTTAATAAAATATCA12.857142857142857No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG12.857142857142857No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA12.857142857142857No Hit
ATCCCAGGACCCTGAGATCATGACGGGAGCTGAAGGCAGACGCTCAACGG12.857142857142857No Hit
CCACAAGAGGGGTCCAGATTGCTTCAAATGAGAATGTGGAAACCATGGAC12.857142857142857No Hit
GATGTATATTCTGAAATGCGAGGCTGGTGTTTATAGCACCCTTGGGTGAT12.857142857142857No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT12.857142857142857No Hit
TGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGACTCATGTTTGTTCAC12.857142857142857No Hit
GATATAAGATTCTTGGTTGACAGTTTCATTCTTTTAGCACTTTGAATATG12.857142857142857No Hit
CATAAAAACCATCTATGAAAAGCCCATAGGCAAATATCATTCTCAATGGA12.857142857142857No Hit
AAGTTACATTTACCCAAATGCAATGGGGCTACACCTCTTAGTTTGAATAG12.857142857142857No Hit
CATGAAGATAACAAGCCAATAGAAGTGGGAACTCGGTCAAAATATGGCAC12.857142857142857No Hit
GGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACCTGAA12.857142857142857No Hit
GAGAAAAATAATGTAAAATATATTAAATATGGAGCTAAGAAAATAACGTA12.857142857142857No Hit
CTTGTGAGCTGGGTTTTCATTTGGTCTCATCAGGCTGACCACTTGGCTGT12.857142857142857No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA12.857142857142857No Hit
GATTATGAGGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGCT12.857142857142857No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG12.857142857142857No Hit
TTCAAGGGACAGCTTGTGGCTCATCTGGTGTTTTAGATTTCTTTACATTT12.857142857142857No Hit
AAGTATATTAACTAGAGAATTGAGTGTAGCTGACAGCATCCTAGTTAGTA12.857142857142857No Hit
AGCACCAACAGTTCGGCATTGTAAGTCCAAATGTCCAGGAAACCATCATC12.857142857142857No Hit
GGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACTTGTCA12.857142857142857No Hit
AGCGAAAGCAGGGGGCTACTTACGATGGCAGCTGACTCACAAGCAAGCTG12.857142857142857No Hit
GCTTTTGGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCA12.857142857142857No Hit
CCTATAAATTCTGCCCACAAATAGCAGGAGGTGTCCCAGTAAAGAATCAG12.857142857142857No Hit
GTAACAGTATTCATAAAGGAACAAAACTTGAAAGCGGTGTGGTGCTGTCT12.857142857142857No Hit
ATCATAGGCAGGAAAAGAAGTTCTACTTCACTTGAAAACTGAAGGAACTG12.857142857142857No Hit
CCATGATTACTGAGCATGAACTACTACCGGAGCAAAACACGCCAGGAAAG12.857142857142857No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)