FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887680
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG76760.8647260273972602No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG38030.42842015140591205No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA25460.2868150684931507No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC25020.28185832732516225No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG17660.19894556596971882No Hit
CTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAA13230.14904019466474405No Hit
TTTCAAATACTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACC13220.14892754145638068No Hit
GCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTC11250.12673485940879595No Hit
ATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAG11230.1265095529920692No Hit
ATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAAACCAA11040.12436914203316511No Hit
GTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCT10240.11535688536409518No Hit
ATATATGCCAAACAAACTTCTATTTTCTAAAGACTCTGGGATTGCTAAGT9850.11096341023792357No Hit
CCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAGGTCACAG9170.10330299206921412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)