FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_G12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_G12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250431
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA41050.32828680670904675No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT39300.31429163224520185No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC37040.296217864080465No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG34860.2787838753197897No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA34440.27542503344846697No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA33910.27118649489655966No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG33540.2682275151527753No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT33530.26814754272726765No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC33260.26598828723856016No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT32270.25807101711329933No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT31340.2506335815410846No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT29970.23967735924653183No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA26620.21288659670145732No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT26560.21240676214841123No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC24400.19513271823875128No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG24130.1929734627500438No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA23860.19081420726133627No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG23660.1892147587511826No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT23400.18713547568798278No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT23170.18529610990130604No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA22660.1812175162004141No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT22490.17985798496678346No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT22240.17785867432909133No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT21240.16986143177832283No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA21230.16978145935281516No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC21060.1684219281191845No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA20900.16714236931106155No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG20420.16330369288669266No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT20370.16290383075915424No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA20270.1621041065040774No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC20220.16170424437653896No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG20050.16034471314290832No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC19990.15986487858986223No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT19950.15954498888783147No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT19900.15914512676029305No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC19810.1584253749307239No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT19730.1577855955266624No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA19720.1577056231011547No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT19620.15690589884607786No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA19260.1540268915278012No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT19140.153067222421709No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT19010.1520275808901091No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT18890.15106791178401685No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC18850.15074802208198615No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT18820.15050810480546306No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC18630.14898862872081706No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC18540.1482688768912479No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC18460.14762909748718642No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA18140.1450699798709405No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC18090.14467011774340208No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA18050.14435022804137135No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT17940.1434705313607868No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT17830.14259083468020226No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA17830.14259083468020226No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT17190.13747259944771043No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT16960.1356332336610337No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC16250.12995519144998804No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG16100.12875560506737277No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG16090.12867563264186507No Hit
GAATTAGCTCATCCTTCAGTTTTGGTGGATTCACATTTAAGAGAACAAGC16080.1285956602163574No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT15950.1275560186847575No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA15810.12643640472764991No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA15690.1254767356215577No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT15560.12443709408995778No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG15450.12355739740937326No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA15400.12315753528183483No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT15340.12267770072878871No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT15330.12259772830328103No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA15130.12099827979312733No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT15050.12035850038906584No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG14910.11923888643195826No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA14820.1185191346023891No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG14820.1185191346023891No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG14680.1173995206452815No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG14660.11723957579426614No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC14620.1169196860922354No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG14620.1169196860922354No Hit
GACAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAAT14570.11651982396469697No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT14480.11580007213512782No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG14410.11524026515657403No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT14330.11460048575251253No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG14300.11436056847598948No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT14250.11396070634845105No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA14250.11396070634845105No Hit
GCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCACAATTATTGC14170.11332092694438958No Hit
GTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCC14170.11332092694438958No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT14160.11324095451888189No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC14080.1126011751148204No Hit
GTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTTGACATAAATCC13860.11084178175365134No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT13800.11036194720060521No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT13720.10972216779654374No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA13600.10876249869045153No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT13540.10828266413740542No Hit
CTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTT13470.10772285715885163No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT13280.10620338107420561No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG13280.10620338107420561No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT13260.10604343622319025No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT13140.10508376711709803No Hit
GATTAATGGTCCTGAATCAGTGTTGGTCAATACCTATCAATGGATCATCA13120.10492382226608266No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA13090.10468390498955961No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT13080.10460393256405193No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA12800.10236470464983674No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA12800.10236470464983674No Hit
GTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTG12760.10204481494780598No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC12690.1014850079692522No Hit
GGATTCAATCGCTGATTCGCCCTATTGACGAAATTCAGATCACCTCTGAC12520.10012547673562155No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT12520.10012547673562155No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)