FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_G11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_G11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841686
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGATTGCCTGTGAGGCCAATGCCTGGGATGTGCAGGCAGCACCT18020.21409409209610236No Hit
GCTTAGGAATGATGAGTGTGATTCTCTTCAGGCCCTGAGCTGTGTCATCC16470.19567867351957857No Hit
GTACTGACTCTGTGGCTTCTGCCTGATGCTCAGCATCGACTGATGTGGGC15740.1870056054157964No Hit
TAATAATACAGGTTGTAAGTAGCAAAACCAGTATTTAAGCACCATCCATT14790.17571873596566892No Hit
TTTCTATACCTCAGCCTTCTTTTCCACAGTCTTTCTAGGTCTTGTCTAAT13210.1569468899328253No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA12060.14328383744056572No Hit
TGTTACTACTGATGCCCTGGGGAGAAGCTGTTAGCCTCTCCTGTAGGTTC11720.13924432626894115No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG10960.13021483070883916No Hit
ATCCTGAGCACTGTGGGGGAAGAACAGGTTCCGGGAGAGGTACTAGGATT10540.12522484632036174No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10460.12427437310350892No Hit
TTACTATACATTGGTTTATTAGGACAAGGTTTCTCATCTTCAGCACTACT9950.11821510634607206No Hit
ATACAGGAGAGCTCCCTACAACAAAAAGTTCCTTGGTCCAAAATACCAGT9220.10954203824228989No Hit
CTTTATATGAGCCCCTTGGAGCAGAACAAGACCATCATTGTGGGCAGAGG9130.10847275587333044No Hit
CTTCAGGCCCTGAGCTGTGTCATCCAGGCACCTTCCCAGAGCATTTAGGT8510.10110658844272093No Hit
GGAATGATGAGTGTGATTCTCTTCAGGCCCTGAGCTGTGTCATCCAGGCA8510.10110658844272093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)