FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834687
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT23850.2857358506841487No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT22650.27135920410884556No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT21030.2519507312321864No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA19340.23170362063863462No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC19100.22882829132357402No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18860.22595296200851336No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA18000.21564969862954614No Hit
CCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGGTCATTGATT17940.214930866300781No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT17460.20918020767065978No Hit
GGATTTATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGA17270.20690390529623678No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA17200.20606526757934412No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT17150.20546624063870647No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA16940.20295032748802846No Hit
GTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCAC16900.20247110593551834No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA16810.2013928574423706No Hit
GATTAACTCCATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGG16450.1970798634697797No Hit
ATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGACA16080.19264706410906124No Hit
CTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCT15450.18509932465702714No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA15220.18234380073009404No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14760.17683275287622785No Hit
TCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCAC14350.17192073196299928No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC14140.16940481881232125No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT13530.16209669013654218No Hit
ACCTAGAAGAGCATCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGA13460.16125805241964952No Hit
GAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT13280.15910155543335405No Hit
GGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGC13210.1582629177164614No Hit
GTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAAGAGTA13170.15778369616395127No Hit
TATCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAA12840.15383011835574295No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT12830.15371031296761542No Hit
CCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAG12600.15095478904068232No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC12390.14843887589000426No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG12260.14688140584434645No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG12060.14448529808179594No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA11770.1410109418260977No Hit
CTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTG11690.14005249872107747No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC11660.1396930825566949No Hit
ATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACC11650.13957327716856738No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA11550.13837522328729213No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG11070.1326245646571709No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC11040.1322651484927883No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA10950.1311868999996406No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC10880.1303482622827479No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT10750.12879079223709006No Hit
ATGTGCAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGA10700.12819176529645246No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA10650.1275927383558148No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT10260.12292032821884132No Hit
ATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATGA10210.12232130127820369No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10060.12052422045629081No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT10020.1200449989037807No Hit
TTCTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAA10020.1200449989037807No Hit
TCCCAGTGCTGGGAAGGACCCTAAGAAAACAGGAGGACCCATATATAGAA9970.11944597196314308No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA9920.11884694502250545No Hit
GTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACCTTCTGGGAAG9740.11669044803620998No Hit
ATCCATCATTGCCCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATAT9720.11645083725995493No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC9670.11585181031931731No Hit
TTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTC9640.11549239415493472No Hit
CCTCTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTT9360.11213784328736401No Hit
GATAATCACTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAA9330.11177842712298143No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT9280.1111794001823438No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT9280.1111794001823438No Hit
GTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGG9120.1092625139723034No Hit
TCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAA9100.10902290319604833No Hit
CAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC8930.1069862115978804No Hit
GTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCA8910.10674660082162536No Hit
TAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAA8820.10566835232847763No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG8720.10447029844720236No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA8680.10399107689469227No Hit
GAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGGAG8650.10363166073030969No Hit
AGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGC8640.10351185534218216No Hit
GAAATAAGGAGAGTTTGGCGCCAAGCAAACAATGGCGAAGATGCAACAGC8530.10219399607277938No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG8390.10051672063899403No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)