FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_C12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_C12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940644
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG21130.22463333631001736No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG19130.20337130731711467No Hit
CTATAGTACTGGTCATACCTATTTTACGAATAGAGAAATGAAGTTTACTT18350.1950791160098826No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12550.13341923193046468No Hit
GTATGATACATGGGGAGGCTCCTTAGGGAGAGTAAGAATTAAATGAACAC12480.13267506091571304No Hit
TTCATAAAGTGATTCATTTGTTAGGAACATACAGCCATCCAGACGCAGTG10530.11194458264763289No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC9950.1057785942396911No Hit
GAGTAGAGAATGAGTAGGACTGAGCTAAATAAAAGGATGGCAGTGGGGTG9940.10567228409472659No Hit
CCTTAGGGAGAGTAAGAATTAAATGAACACACAGAGTAGAGAATGAGTAG9580.10184511887600411No Hit
CTATTAATATAGGGCAACATTTCCCAAAGCGTGGTATGCTGAACGCTGAC9410.10003784641160736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)