FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_C11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_C11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809774
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG39460.48729645555426576No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG18880.23315147189215757No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14880.1837549736099208No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG14160.17486360391911818No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC12730.15720435578321854No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA12000.14818949484671032No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA11540.14250889754425308No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG9520.11756366591172353No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA9310.11497034975190609No Hit
ATCTTATACCATGAGCATTTTCCCACAATCATGTGCTATCCCTCCCTTAC8840.10916626120374326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)