FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_A09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_A09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260645
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA24800.19672469251851235No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA23430.18585723974632035No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT23320.1849846705456334No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT22970.1822083139979931No Hit
GAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAACCTT22920.18181169163404448No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC22690.17998722875988085No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT21930.17395856882786193No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC20480.16245652027335214No Hit
CTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATCATTG18970.150478524882104No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA18950.15031987593652457No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC18240.14468783836845425No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC18200.14437054047729533No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18190.14429121600450565No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC18140.143894593640557No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC17560.1392937742187531No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA17420.13818323159969698No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT17140.13596214636158474No Hit
GGCAATGAAAGAGTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTG16950.13445498137858No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA16870.13382038559626222No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA16230.12874361933771997No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG16110.1277917256642433No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG16040.12723645435471523No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC15840.1256499648989208No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC15540.1232702307152291No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA14650.11621035263694379No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT14040.1113715597967707No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA13880.11010236823213514No Hit
GGCATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC13810.10954709692260708No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT13480.10692938932054623No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG13440.10661209142938732No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA13200.10470830408243399No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG12640.10026613360620952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)