FastQCFastQC Report
Thu 20 May 2021
HHV25BGXJ_n01_S11_R1_A08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHV25BGXJ_n01_S11_R1_A08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008233
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA21330.2115582410018319No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC19800.19638317730127858No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC17000.16861181889503715No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT15730.15601552418934908No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA15420.15294083808008666No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA15230.1510563530453774No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC15100.14976696854794477No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC14210.14093964391167516No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG14090.13974944283712198No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC13670.13558373907618576No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAGTCATGG13400.13290578665844105No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC13130.13022783424069634No Hit
CTATTAATAGTGGCCGGATTTTTTCAATTTTCTTTCTTGTTGAATCATTG12900.12794661551446937No Hit
CTCCTATTGTGACTGGGTGTACATTCTGGAAAGGAAGACTACTGTTTATA12780.12675641443991617No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG12110.12011112510699412No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG11980.1188217406095615No Hit
GTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGATGTT11830.11733398926636997No Hit
CTTGTATATGGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGGGCTGGGAT11560.11465603684862526No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG11440.11346583577407207No Hit
ATATAGTGGTATTCCCTCTCCTTCATGACTTGGATCCTCAATGCTTAATT11360.11267236839103659No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC11250.11158135073936282No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA11040.10949849885889473No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT10820.10731646355554719No Hit
GTCAAAAATCACACTGAAGTTCGCTTTCAGTATGATGTTCTTATCCATTA10510.10424177744628473No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG10290.10205974214293718No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA10230.1014646416056606No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT10220.10136545818278116No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT10200.10116709133702229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)