FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19651676
Sequences flagged as poor quality0
Sequence length55
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4657642.37009810257405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACAT435770.2217469899259483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCG263580.13412596462510373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGGCATTGAGGCAGCGAGCGC209380.10654561982397838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1499850.046.2081071
AGCAGTG1626950.042.4890982
GCAGTGG2065650.033.552683
AGTGGTA2198600.031.6369535
CAGTGGT2244850.030.7955324
GTGGTAT2404700.028.897776
TGGTATC2414700.028.5719767
GTATCAA2437250.028.3197489
GGTATCA2440750.028.2428558
ATCAACG2456500.028.13245211
CAACGCA2489300.027.67501613
TATCAAC2516000.027.5189110
AGAGTAC2497850.027.34878319
CAGAGTA2521250.027.26232118
GAGTACA2497750.027.25622420
AACGCAG2541850.027.2068314
CGCAGAG2552350.027.16821516
AGTACAT2486750.027.12013621
GCAGAGT2543900.027.0681617
TACATGG2445350.027.05908823
Produced by FastQC (version 0.11.5)