FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA291_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA291_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59172880
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6143600.046.9158631
AGCAGTG6797250.042.3142432
GCAGTGG8078900.035.536233
CAGTGGT8626100.033.2753224
AGTGGTA8912700.032.2768065
GTGGTAT9710800.029.6035986
TGGTATC9703250.029.5617337
GGTATCA9923800.028.9098458
GTATCAA9988050.028.7434779
TATCAAC10021900.028.64198710
ATCAACG10037600.028.5630411
TCAACGC10025250.028.52556812
CAACGCA10066700.028.41319313
AACGCAG10147500.028.23195614
ACGCAGA10175000.028.1554815
CGCAGAG10219700.028.02548816
AGAGTAC10099900.027.86738819
GAGTACA10106650.027.75782420
GCAGAGT10236350.027.73369817
CAGAGTA10225100.027.66492818
Produced by FastQC (version 0.11.5)