FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA291_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA291_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67356337
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6997250.046.900871
AGCAGTG7741300.042.3192442
GCAGTGG9204750.035.5553473
CAGTGGT9836500.033.2517934
AGTGGTA10169850.032.228475
GTGGTAT11078250.029.557086
TGGTATC11061350.029.5209437
GGTATCA11303800.028.8915048
GTATCAA11385450.028.6933049
TATCAAC11416400.028.60615510
ATCAACG11433150.028.54478611
TCAACGC11425400.028.51390812
CAACGCA11469800.028.40633813
AACGCAG11561900.028.22608614
ACGCAGA11601950.028.13170815
CGCAGAG11649400.028.005416
AGAGTAC11499100.027.82216619
GAGTACA11509550.027.7202220
GCAGAGT11674250.027.70447317
CAGAGTA11646300.027.64706418
Produced by FastQC (version 0.11.5)