FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52067547
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5446450.046.908141
AGCAGTG6018650.042.3825872
GCAGTGG7139750.035.671133
CAGTGGT7628050.033.367164
AGTGGTA7870400.032.4042975
GTGGTAT8578700.029.7040186
TGGTATC8564300.029.6817557
GGTATCA8755950.029.0335678
GTATCAA8815750.028.8498529
TATCAAC8836150.028.77955610
ATCAACG8859250.028.68524211
TCAACGC8854250.028.64696712
CAACGCA8887900.028.54425213
AACGCAG8961250.028.35337314
ACGCAGA8988300.028.26815215
CGCAGAG9027400.028.13387916
AGAGTAC8913700.027.9466719
GCAGAGT9042500.027.84577217
GAGTACA8924650.027.82221820
CAGAGTA9027450.027.76356518
Produced by FastQC (version 0.11.5)