FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57736789
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6020950.046.9317671
AGCAGTG6664850.042.316242
GCAGTGG7935400.035.4892923
CAGTGGT8474450.033.218534
AGTGGTA8740450.032.264515
GTGGTAT9526100.029.5863446
TGGTATC9515700.029.5551327
GGTATCA9730600.028.9106738
GTATCAA9798050.028.730439
TATCAAC9823150.028.65534810
ATCAACG9833350.028.59355511
TCAACGC9830800.028.53663812
CAACGCA9869600.028.42989313
AACGCAG9944050.028.24769214
ACGCAGA9974500.028.16140715
CGCAGAG10019750.028.02791616
AGAGTAC9899500.027.84390819
GAGTACA9909300.027.73593720
GCAGAGT10038700.027.73576717
CAGAGTA10024200.027.66828718
Produced by FastQC (version 0.11.5)