FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA290_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA290_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62485955
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACAT5079520.8129058762085016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCG2993900.4791316704689878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGGCATTGAGGCAGCGAGCGC2395700.3833981572338936No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACATAA1155300.1848895483793118No Hit
GTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACATAACTA806780.12911381445638465No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCGCA764070.12227867846462458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9092400.047.5392881
AGCAGTG10199550.042.3234142
GCAGTGG13207700.032.664323
AGTGGTA13733550.031.4358335
CAGTGGT14687450.029.4041984
GTGGTAT15116150.028.5299076
TGGTATC15089850.028.5066747
GGTATCA15452100.027.8321578
GTATCAA15579700.027.6020769
TATCAAC15628000.027.50092310
ATCAACG15745300.027.27822111
TCAACGC15772950.027.17628112
CAACGCA15851250.027.04594613
AACGCAG15993350.026.85543414
ACGCAGA16030400.026.79193915
AGAGTAC15987150.026.51669519
GCAGAGT16123150.026.479917
CAGAGTA16096100.026.4543518
GAGTACA15994200.026.44253220
AGTACAT15929800.026.3521921