FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA290_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA290_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60632574
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACAT4880580.804943560535629No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCG2894890.47744798035458635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGGCATTGAGGCAGCGAGCGC2321250.38283876914082515No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACATAA1123830.18535086437201231No Hit
GTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACATAACTA773370.1275502504643791No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCGCA743420.12261066139135048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8813150.047.5377581
AGCAGTG9867300.042.393532
GCAGTGG12782000.032.7415163
AGTGGTA13283450.031.4931765
CAGTGGT14208950.029.467544
GTGGTAT14624850.028.5739546
TGGTATC14594750.028.5597067
GGTATCA14929950.027.9109048
GTATCAA15032350.027.7050869
TATCAAC15080550.027.6037610
ATCAACG15203400.027.36740511
TCAACGC15230200.027.27373112
CAACGCA15304900.027.14675913
AACGCAG15444400.026.94436814
ACGCAGA15477200.026.88653415
AGAGTAC15462000.026.60051319
GCAGAGT15596150.026.55980517
CAGAGTA15566000.026.54041118
GAGTACA15463900.026.53691120
AGTACAT15397700.026.44463521