FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n02_CA290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n02_CA290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58978851
Sequences flagged as poor quality0
Sequence length55
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACAT4784580.8112365566429907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCG2842160.48189477275506776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCAGGCATTGAGGCAGCGAGCGC2282420.38698956681946894No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACATAA1098170.18619725229981168No Hit
GTGGTATCAACGCAGAGTACATGGGGGACTTCCCGTGTGGCTCACAACATAACTA764280.12958543393800603No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCAGGCATTGAGGCAGCGAGCGCA729140.12362736601972799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8571450.047.5227551
AGCAGTG9598550.042.3729862
GCAGTGG12431850.032.7045973
AGTGGTA12925600.031.4490285
CAGTGGT13824300.029.4353144
GTGGTAT14228050.028.5403066
TGGTATC14205850.028.5116677
GGTATCA14538500.027.8518948
GTATCAA14662900.027.625299
TATCAAC14708600.027.52959310
ATCAACG14824450.027.29488211
TCAACGC14851600.027.19790812
CAACGCA14918800.027.07696513
AACGCAG15053100.026.87324314
ACGCAGA15087350.026.8131815
AGAGTAC15045950.026.54852719
GCAGAGT15177850.026.49729517
CAGAGTA15155500.026.47890918
GAGTACA15052000.026.47842220
AGTACAT15001200.026.3724621