FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19651676
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG3098381.5766492384669886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA20150.017.1211011
GGGACAA26850.016.5843891
ATGGGAG17200.016.276751
GAGACCC26150.015.848151
TGCGACG19550.015.6169221
GAAAAGG13900.015.42950254
ATCGGCG18000.015.3084051
CTGAAAG19350.015.2329783
AGGCGAA15600.015.2286458
CCGGTGA23250.015.2175431
TAGCGTG15300.015.1678967
GCGAGAC9600.015.12288955
GGGATAG14650.015.0900063
GGAGGGA10700.015.0072526
GGAGGAT28350.014.9293381
AGGGAAG11650.014.9163353
CCGGACA17050.014.8684561
GATGACT27850.014.8016051
CGAATTG23850.014.603641
GGGCTAC17200.014.4824631
Produced by FastQC (version 0.11.5)