FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA291_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA291_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59172880
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG102250.017.3036461
AGGCGAA71500.016.8918638
GCAAGAG116350.016.7808367
AGGATAC114600.016.7587228
GAAAAGG87400.016.7009054
AGGAACG68050.016.6490868
CCCGAAG123000.016.6477741
AGACGCC78100.016.4784418
GAGACCC118800.016.4305481
AGCGCCG65550.016.3611288
ACGCGAG80100.016.3553353
AAGGAAG89250.016.3300613
CACGGAG81100.016.221483
CTGAAAG98350.016.2059383
AACAGGG111200.016.1385331
ATGGGAG80850.016.1269871
AAAAGCG48600.016.1148035
GGAAGCA89200.016.0432593
AGACGGA79500.015.9530418
CGGACAC94450.015.9250741
Produced by FastQC (version 0.11.5)