FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA291_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA291_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67356337
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG117650.017.5729941
AGGATAC130900.016.75598
AGGCGAA84950.016.63888
GAAAAGG93350.016.5201264
GAGACCC133000.016.47141
CCCGAAG132150.016.3608671
CGGGAGG58550.016.3445134
AGGCGCT95850.016.2844288
GCAAGAG129250.016.2634037
AGCGCCG72850.016.2617918
GCGCCGT73450.016.1289399
AGGAACG77550.016.1272728
AGACGGA89500.016.1124618
GAGACGA61600.016.053157
CGGACAC106650.015.8983741
AACAGGG120750.015.8916971
AGACGCC90950.015.8676788
GTGCGTG140150.015.8352051
AAAAGCG56550.015.8333375
GGAAGCA103300.015.7913723
Produced by FastQC (version 0.11.5)