FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52067547
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG89450.017.5778871
AGCGCCG56700.016.8003928
AGGCGAA66250.016.7694998
AGGATAC101900.016.6778078
GAAAAGG74000.016.6035214
CCCGAAG104200.016.5785561
AGGCGCT73550.016.3912648
AGGAACG60000.016.353038
ACGCGAG69900.016.3342253
GCAAGAG97600.016.229167
CGGACAC83400.016.214391
GAGACCC104950.016.156041
CACGGAG70700.016.1338393
AGACGAA68600.016.1308968
GCGCCGT59550.016.0517129
GCGAAAC61050.016.0176759
AAAGACG53650.015.97162156
GGAAGCA81150.015.9403243
AACAGGG96950.015.9002921
CTGAAAG90850.015.8850013
Produced by FastQC (version 0.11.5)