FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57736789
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG96800.017.5044361
GAAAAGG82350.016.9770874
AGGCGAA76800.016.814728
GAGACCC122400.016.6480081
GACGCGA49050.016.595462
GCAAGAG111350.016.5861177
AGGATAC109600.016.5698768
ACGCGAG79700.016.4650463
GGAAGCA86800.016.2461013
CCCGAAG115350.016.2156371
AGACGGA83300.016.1364848
CGGACAC95650.016.1044461
CACGGAG81450.016.070773
AGCGCCG64500.016.0476958
CGGGAGG50800.015.95824054
ATGGGAG78950.015.9432421
CGTGATA100750.015.9226451
AGACGCC78400.015.8963198
AGGAACG64800.015.8715518
AAAGACG60550.015.8229796
Produced by FastQC (version 0.11.5)