FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA290_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA290_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62485955
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACAA151300.018.3482281
GCCCGAA124450.018.0364481
TGCGACG127100.017.4699381
CCCGAAG121500.017.3423631
CCGGTGA122250.017.0829581
AGACATA203850.017.008118
TAACGAC184950.016.9731791
GCGGAAA101300.016.5535551
GGAGGAT137750.016.5506061
AAACGAA131500.016.4837591
TGGGAAG125600.016.3031861
GGCGAGA57600.016.2893834
CGGGCGT77000.016.2851433
CTGGACG92300.016.2245331
CGAGGAA86600.016.2124461
GCGACGT71050.016.17882
TGTGGCG100650.016.1357211
TCACGGA130050.016.0280048
CGAGGAC74900.016.0222246
GGCACGT157100.016.0109021
Produced by FastQC (version 0.11.5)