FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA290_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA290_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60632574
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACAA147000.018.3311421
GCCCGAA121550.017.561631
CCCGAAG119550.017.4688661
CCGGTGA125200.017.3836141
AGACATA197850.017.3793778
TGCGACG124300.017.3058821
GCGGAAA100150.016.9414861
TAACGAC179550.016.919951
GGAGGAT140450.016.702581
CGGGCGT72900.016.5674253
CGAGGAA87900.016.5485761
AAACGAA133400.016.537761
GGCGAGA56250.016.3479864
GGCACGT158700.016.1962341
AGGGAGG78050.016.1648548
CTGGACG87900.016.1480061
GCGACGT66700.016.1455212
CATGACG115250.016.1010958
CGAGGAC77450.015.9634936
TCACGGA119050.015.9290238
Produced by FastQC (version 0.11.5)