FastQCFastQC Report
Wed 5 Jun 2019
HHTNVBGXB_n01_CA290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTNVBGXB_n01_CA290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57323031
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACAA145750.018.4954591
GCCCGAA118600.017.8495771
CCCGAAG111700.017.7602921
TGCGACG116850.017.4483451
CCGGTGA116950.017.3675671
AGACATA187950.017.3407738
TAACGAC169250.017.2861081
GGAGGAT137000.016.8095551
GCGGAAA93150.016.5722851
GGCGAGA52150.016.4098854
CATGACG111450.016.344048
GGCACGT144000.016.3439061
AAACGAA123450.016.2748581
GCCGTGA103800.016.101561
CTGGACG83250.015.9922191
TCACGGA117250.015.9858538
CGAGGAA85850.015.9692791
ACATGCA142350.015.96136951
TGGGAAG119400.015.8869491
GCGACGT62250.015.7978692
Produced by FastQC (version 0.11.5)