FastQCFastQC Report
Thu 30 Mar 2017
HHTJGBCXY_l02n03.33300000083c1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTJGBCXY_l02n03.33300000083c1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163100018
Sequences flagged as poor quality0
Sequence length99
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGATCCCTGGGTGGCGCAG3099270.19002266449780525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17234800.076.7615661
AGCAGTG20203350.065.056292
GCAGTGG27001050.048.5409473
CAGTGGT28026200.046.7214554
AGTGGTA28404900.046.072515
GTGGTAT30503650.042.857376
TGGTATC30497550.042.7817427
GGTATCA30900250.042.288128
GTATCAA31238150.042.080939
ATCAACG31213250.025.08211310-11
CAACGCA31224000.025.0041712-13
ACGCAGA31476700.024.74736614-15
AGAGTAC31127250.024.66131618-19
TACATGG29989900.024.65070322-23
AGTACAT30958050.024.49745620-21
GCAGAGT31584850.024.35011516-17
CATGGGG18882300.023.67647724-25
AGTCGTA136150.023.6526935
CATGGGA5980900.022.29628424-25
CATGGGC4859900.021.71339624-25
Produced by FastQC (version 0.11.2)