FastQCFastQC Report
Thu 30 Mar 2017
HHTJGBCXY_l01n03.33300000083be3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTJGBCXY_l01n03.33300000083be3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160988576
Sequences flagged as poor quality0
Sequence length99
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGATCCCTGGGTGGCGCAG3967110.24642183306224164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT14117050.078.3686751
AGCAGTG16435850.066.724372
GCAGTGG22496700.048.6057133
CAGTGGT23563500.046.3553354
AGTGGTA23757500.045.8910375
GTGGTAT26334300.041.3797076
TGGTATC26315800.041.2902957
GGTATCA26757250.040.664048
GTATCAA27135800.040.3923239
AGTCGTA133350.024.0597275
ATCAACG27234400.023.8113810-11
CAACGCA27267850.023.7155412-13
ACGCAGA27518550.023.46670514-15
AGAGTAC27288250.023.40596818-19
TACATGG26528400.023.30856722-23
AGTACAT27260750.023.2110120-21
GCAGAGT27763450.023.02980416-17
CATGGGG17072300.022.36333324-25
CATGGGA5193200.020.6368424-25
CGCGATC1088700.020.56391178-79
Produced by FastQC (version 0.11.2)