FastQCFastQC Report
Thu 30 Mar 2017
HHTJGBCXY_l01n01.33100000083be9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHTJGBCXY_l01n01.33100000083be9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160988576
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC526900.017.9278581
CCTTACG552950.017.806241
GACTAAC431200.017.6712971
CGCTATC463800.017.6404651
TTTACTG387450.017.543361
TGATTTC474850.017.5379161
GGATTAC466750.017.5357341
CTTTGCG507200.017.4979041
TGCTACC478800.017.4020231
CCTACCA527350.017.3918821
ACTGAAC523450.017.2883241
CGCTTCA379800.017.2817881
TACCTTA572300.017.2354451
CATATTC455600.017.1682451
GATGCTA438800.017.1566471
CTGCCTA449150.017.145431
ACTGATG384050.017.1398531
GCGCGAT524750.017.1111721
CCCTCCT434300.017.0824891
ATAGACC562750.017.0773981
Produced by FastQC (version 0.11.2)